PXD003398

PXD003398 is an original dataset announced via ProteomeXchange.

Title	Global quantitative proteomes of PrP-deficient mouse models
Description	A popular method for studying the function of a given protein is to generate and characterize a suitable model deficient for its expression. Most researchers select mouse models for this purpose and investigate proteins of interest in the tissue they are most associated with. For the prion protein (PrP), best known for its role in several invariably fatal neurodegenerative diseases of humans and animals, a natural choice, therefore, would be to undertake such studies with mouse brain samples. We recently documented the surprising observation that PrP deficiency caused a loss or enhancement of NCAM1 polysialylation, dependent on the cell model used. To identify possible causes for this disparity, we set out to systematically investigate the consequence of PrP deficiency on the global proteome in brain tissue and in four distinct cell models. Here we report that PrP deficiency has no discernible impact on the global brain proteome but causes robust but surprisingly divergent changes to the global proteomes of cell models. Our data suggest that careful investigations in cell models, rather than mouse tissue, are the way forward for elucidating the molecular function of PrP.
HostingRepository	PRIDE
AnnounceDate	2016-06-01
AnnouncementXML	Submission_2016-06-01_03:34:17.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gerold Schmitt-Ulms
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	deamidated residue; S-pyridylethyl-L-cysteine; TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2016-01-04 03:17:01	ID requested
⏵ 1	2016-06-01 03:34:17	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: prion protein, mouse, global proteome, mass spectrometry

Gerold Schmitt-Ulms
contact affiliation	Tanz Centre for Research in Neurodegenerative Diseases, University of Toronto, Ontario M5T2S8, Canada. Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario M5S1A8, Canada
contact email	g.schmittulms@utoronto.ca
lab head
Gerold Schmitt-Ulms
contact affiliation	University of Toronto
contact email	g.schmittulms@utoronto.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/06/PXD003398

PRIDE project URI

• PRIDE
  1. PXD003398
     1. Label: PRIDE project
     2. Name: Global quantitative proteomes of PrP-deficient mouse models