PXD003385

PXD003385 is an original dataset announced via ProteomeXchange.

Title	Characterisation and use of orthotopically grownHapT1tumours in its homologous host as a preclinical model to evaluate novel drugs against desmoplastic pancreatic cancer
Description	The major objective of this study was to characterise theHapT1 orthotopic hamster pancreatic tumor as a preclinical model of desmoplastic pancreatic ductal adenocarcinoma; and use this model to validate the efficacy of drugs that kills activated pancreatic stellate cells (PSCs)in vitro. Experimentaldesign: Commercially procured HapT1 pancreatic cancer (PCA) cell line was implanted in the pancreas of its syngeneic host, Syrian golden hamster (Mesocricetusauratus). After certain time period the primary and secondary tumors were harvested for histological andimmunophynotypical analysis. PSCs of hamsters were harvested, cultured and characterised. The in-cultured activated rodent PSCs and commercially procured human PSCs were used to check the cytotoxic effect of Disulfiram (DSF) in presence and absence of copper (Cu )in vitro. Finally, theHapT1 orthotopic tumor model was used to check the efficacy of DSF in vivo.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:27:33.163.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shantibhusan Senapati
SpeciesList	scientific name: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus); NCBI TaxID: 10029;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Bruker Daltonics maXis series

Revision	Datetime	Status	ChangeLog Entry
0	2015-12-23 00:33:57	ID requested
1	2018-10-26 05:35:54	announced
⏵ 2	2024-10-22 04:27:35	announced	2024-10-22: Updated project metadata.

10.18632/oncotarget.9729;

Suklabaidya S, Das B, Ali SA, Jain S, Swaminathan S, Mohanty AK, Panda SK, Dash P, Chakraborty S, Batra SK, Senapati S, Characterization and use of HapT1-derived homologous tumors as a preclinical model to evaluate therapeutic efficacy of drugs against pancreatic tumor desmoplasia. Oncotarget, 7(27):41825-41842(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: pancreatic cell line,Hamster, LC-MS/MS

Dr. Shantibhusan Senapati
contact affiliation	Institute of life sciences (ILS) Bhubaneswar National dairy Research Institute (NDRI) Haryana, INDIA
contact email	drsantibhusan@yahoo.com
lab head
Shantibhusan Senapati
contact affiliation	Institute of Life Sciences
contact email	drsantibhusan@yahoo.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003385
     1. Label: PRIDE project
     2. Name: Characterisation and use of orthotopically grownHapT1tumours in its homologous host as a preclinical model to evaluate novel drugs against desmoplastic pancreatic cancer