PXD003383

PXD003383 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of TRAIL-induced endogenous RIPK3-dependent necrosome complex
Description	Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a well-known inducer of apoptosis via formation of the primary death-inducing signaling complex (TRAIL-DISC) at the level of membrane death receptors (DR4 and DR5) which recruit successively FADD and caspase-8. TRAIL can also induce necroptosis when caspases are inhibited. Necroptosis is a regulated cell death dependent on the formation of a cytosolic necrosome complex which includes RIPK1, RIPK3 and MLKL proteins. Elucidating the molecular mechanisms involved in TRAIL-induced necroptosis might provide new insights into the TRAIL death signaling pathway. Here, we report the analysis by mass spectrometry of endogenous RIPK3-dependent necrosome complex constituents upon necroptosis induced by TRAIL/z-VAD/Birinapant (TzB) in HT29 cells. Besides characterization of RIPK1, RIPK3, MLKL, FADD, caspase-8, we find TRIM21 as a new constituent of the necrosome complex. Moreover RIPK1, RIPK3, MLKL, P-MLKL, FADD, caspase-8 and TRIM21 are also found associated to the native TRAIL-DISC upon TzB stimulation showing initiation of the necrotic pathway at the level of TRAIL death receptors in HT29 cells. Finally, TRIM21 may positively modulate necroptosis induction by downregulating NF-kB activation.
HostingRepository	PRIDE
AnnounceDate	2021-03-18
AnnouncementXML	Submission_2021-03-18_07:48:25.113.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003383
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Melanie Lagarrigue
SpeciesList	scientific name: Colon; NCBI TaxID: 1542223; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide - site C; monohydroxylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-12-21 08:03:51	ID requested
⏵ 1	2021-03-18 07:48:25	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: RIPK3-dependent necrosome

DISC

mass spectrometry

TRAIL

TRIM21

Charles Pineau
contact affiliation	Protim, Inserm U1085 Irset
contact email	charles.pineau@inserm.fr
lab head
Melanie Lagarrigue
contact affiliation	Life Sciences
contact email	melanie.lagarrigue@univ-rennes1.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003383
     1. Label: PRIDE project
     2. Name: Proteomic analysis of TRAIL-induced endogenous RIPK3-dependent necrosome complex