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PXD003382

PXD003382 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCEP215_gallus_interactome
DescriptionTo establish the molecular basis for CEP215 function in centrosome-spindle pole attachment, we employed an unbiased proteomic approach to isolat e and identify CEP215 interactors. To this end, affinity purification tags ( Gs TAP containing protein G and streptavidin-binding protein) were inserted in-frame into both alleles of the CEP215 gene (CEP215-TAP cell line) in the chicken B cell line, DT40. Following affinity purification, protein complexes were analysed by mass spectr ometry
HostingRepositoryPRIDE
AnnounceDate2016-03-31
AnnouncementXMLSubmission_2016-03-31_07:04:08.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPavithra Lakshminarasimhan
SpeciesList scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031;
ModificationListmonohydroxylated residue; deamidated residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-12-21 05:52:57ID requested
12016-03-31 07:04:09announced
Publication List
Chavali PL, Chandrasekaran G, Barr AR, T, รก, trai P, Taylor C, Papachristou EK, Woods CG, Chavali S, Gergely F, A CEP215-HSET complex links centrosomes with spindle poles and drives centrosome clustering in cancer. Nat Commun, 7():11005(2016) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: mitosis, centrosome, centrosome clustering, cancer, micr ocephaly, CEP215, HSET,
Contact List
Fanni gergely
contact affiliationCancer Research UK Cambridge Institute, Li Ka Shing Centre , University of Cambridge, Robinson Way, Cambridge CB2 0RE, UK
contact emailfanni.gergely@cruk.cam.ac.uk
lab head
Pavithra Lakshminarasimhan
contact affiliationUniversity of cambridge
contact emailpavithra.lakshminarasimhan@cruk.cam.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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