PXD003362 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | microRNA-140 inhibits inflammation and stimulates chondrogenesis in a model of IL1β-induced osteoarthritis |
Description | Osteoarthritis is a devastating disease of articular cartilage. The pathogenic factors contributing to this disorder are inflammation, extracellular matrix degradation and failure to rebuild the articular cartilage. Preclinical studies suggest that microRNA-140 (miR-140) may play a protective role in osteoarthritis development, but little is known about the mechanism by which this may occur. Here we present the results of overexpression of miR-140 in an in vitro model of osteoarthritis, evaluated by global proteomics analysis. We show that inflammation is reduced through the differential synthesis of multiple proteins involved in the nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (NFKB) pathway, articular cartilage regeneration may be induced through the upregulation of many of the components involved in the synthesis of hyaline extracellular matrix, and cartilage degeneration may be reduced through the reduced expression of aggrecanases. Thus, miR-140 overexpression may have a beneficial effect on the development of osteoarthritis through the differential synthesis of a surprisingly large number of proteins involved in all three pathogenic processes. These results show how intraarticular injection of miR-140 may benefit patients suffering from osteoarthritis. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:28:05.353.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Gustavo de Souza |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-12-16 23:52:58 | ID requested | |
1 | 2018-10-26 04:28:30 | announced | |
⏵ 2 | 2024-10-22 04:28:13 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1038/mtna.2016.64; |
Karlsen TA, de Souza GA, Ø, degaard B, Engebretsen L, Brinchmann JE, -induced Osteoarthritis. Mol Ther Nucleic Acids, 5(10):e373(2016) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: IL1B, osteoarthritis,microRNA-140, inflammation, articular cartilage |
Contact List
Jan Brinchmann |
contact affiliation | Department of Immunology, Oslo University Hospital Rikshospitalet, PO Box 4950 Nydalen, 0424 Oslo, Norway |
contact email | Jan.Brinchmann@rr-research.no |
lab head | |
Gustavo de Souza |
contact affiliation | Dept. of Immunology |
contact email | g.a.d.souza@medisin.uio.no |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003362
- Label: PRIDE project
- Name: microRNA-140 inhibits inflammation and stimulates chondrogenesis in a model of IL1β-induced osteoarthritis