PXD003362

PXD003362 is an original dataset announced via ProteomeXchange.

Title	microRNA-140 inhibits inflammation and stimulates chondrogenesis in a model of IL1β-induced osteoarthritis
Description	Osteoarthritis is a devastating disease of articular cartilage. The pathogenic factors contributing to this disorder are inflammation, extracellular matrix degradation and failure to rebuild the articular cartilage. Preclinical studies suggest that microRNA-140 (miR-140) may play a protective role in osteoarthritis development, but little is known about the mechanism by which this may occur. Here we present the results of overexpression of miR-140 in an in vitro model of osteoarthritis, evaluated by global proteomics analysis. We show that inflammation is reduced through the differential synthesis of multiple proteins involved in the nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (NFKB) pathway, articular cartilage regeneration may be induced through the upregulation of many of the components involved in the synthesis of hyaline extracellular matrix, and cartilage degeneration may be reduced through the reduced expression of aggrecanases. Thus, miR-140 overexpression may have a beneficial effect on the development of osteoarthritis through the differential synthesis of a surprisingly large number of proteins involved in all three pathogenic processes. These results show how intraarticular injection of miR-140 may benefit patients suffering from osteoarthritis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:28:05.353.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gustavo de Souza
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-12-16 23:52:58	ID requested
1	2018-10-26 04:28:30	announced
⏵ 2	2024-10-22 04:28:13	announced	2024-10-22: Updated project metadata.

10.1038/mtna.2016.64;

Karlsen TA, de Souza GA, Ø, degaard B, Engebretsen L, Brinchmann JE, -induced Osteoarthritis. Mol Ther Nucleic Acids, 5(10):e373(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: IL1B, osteoarthritis,microRNA-140, inflammation, articular cartilage

Jan Brinchmann
contact affiliation	Department of Immunology, Oslo University Hospital Rikshospitalet, PO Box 4950 Nydalen, 0424 Oslo, Norway
contact email	Jan.Brinchmann@rr-research.no
lab head
Gustavo de Souza
contact affiliation	Dept. of Immunology
contact email	g.a.d.souza@medisin.uio.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD003362

PRIDE project URI

• PRIDE
  1. PXD003362
     1. Label: PRIDE project
     2. Name: microRNA-140 inhibits inflammation and stimulates chondrogenesis in a model of IL1β-induced osteoarthritis