PXD003348

PXD003348 is an original dataset announced via ProteomeXchange.

Title	Quantification of sulfenic acid on model protein
Description	Aldolase contains eight free cysteine residues without disulfide formation in its native state, which makes it a suitable model to manipulate the oxidative modifications on cysteine. H2O2 was chosen to treat aldolase since the principle product between which is sulfenic acid.To assess the reduction of the newly formed sulfenic acids on aldolase by arsenite, a differential alkylation strategy was adapted.To further investigate the formation of other reversible cysteine oxidations such as disulfide on aldolase, arsenite was replaced by DTT in the differential alkylation method to reduce all the reversible cysteine oxidations.To further validate the selectivity of the reducing ability of arsenite on sulfenic acid but not other oxidative modifications especially disulfide, lysozyme was employed in the differential alkylation method. Lysozyme has 8 cysteine residues all bound into disulfide bonds, thereby was not treated with H2O2 to avoid further oxidation.
HostingRepository	PRIDE
AnnounceDate	2016-10-10
AnnouncementXML	Submission_2016-10-10_03:36:18.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003348
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ru Li
SpeciesList	scientific name: Mammalia; NCBI TaxID: 40674; scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031; scientific name: Lagomorpha; NCBI TaxID: 9975;
ModificationList	N-ethylmaleimide derivatized cysteine; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-12-15 02:28:47	ID requested
⏵ 1	2016-10-10 03:36:19	announced

Li R, Klockenbusch C, Lin L, Jiang H, Lin S, Kast J, on Monocytes via NADPH Oxidase. J Proteome Res, 15(12):4221-4233(2016) [pubmed]

curator keyword: Biological

submitter keyword: sulfenic acid, aldolase, lysozyme, arsenite

Juergen Kast
contact affiliation	Professor, University of British Columbia
contact email	kast@brc.ubc.ca
lab head
Ru Li
contact affiliation	Univerisity of British Columbia
contact email	rli@brc.ubc.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003348
     1. Label: PRIDE project
     2. Name: Quantification of sulfenic acid on model protein