PXD003335

PXD003335 is an original dataset announced via ProteomeXchange.

Title	Chicken plasma proteome LPS
Description	Lipopolysaccharides (LPS) are cell wall components of gram-negative bacteria and endotoxins that cause inflammation and sickness in vertebrate animals. Our objective to identify the plasma proteomic changes in an avian model of inflammation. Chickens were treated with either saline or LPS and the blood was collected 24 hours after injection. The pooled plasma samples were depleted of high abundant proteins, analyzed by Matrix assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF-MS) and liquid chromatography–tandem mass spectrometry (LC-MS/MS). MALDI-TOF analyses showed an increase in fibrinogen beta derived peptide and decrease in apolipoprotein AII- isoform X1 derived peptide in LPS samples. Label free quantitation of LC-MS/MS spectra revealed an increase in alpha-1 acid glycoprotein, chemokine-CCLI10, and cathelicidin-2 and decrease in interferon stimulated gene-12-2 in the LPS group. These differentially expressed proteins are associated with inflammation, cytokines, immunomodulation, and defense which are useful as candidate biomarkers of infection and inflammation.
HostingRepository	PRIDE
AnnounceDate	2016-08-09
AnnouncementXML	Submission_2016-08-09_02:38:18.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003335
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Balamurugan Packialakshmi
SpeciesList	scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031;
ModificationList	dihydroxylated residue; sulfated residue; dehydrated residue; phosphorylated residue; trifluoroacetic acid adduct; monohydroxylated residue; acetylated residue; deaminated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Bruker Daltonics amaZon series

Revision	Datetime	Status	ChangeLog Entry
0	2015-12-14 03:06:02	ID requested
⏵ 1	2016-08-09 02:38:19	announced

Packialakshmi B, Liyanage R, Lay JO, Makkar SK, Rath NC, Proteomic Changes in Chicken Plasma Induced by Salmonella typhimurium Lipopolysaccharides. Proteomics Insights, 7():1-9(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: endotoxin, biomarkers, innate immunity, acute phase proteins

Narayan C Rath
contact affiliation	PPPSRU USDA ARS
contact email	narayan.rath@ars.usda.gov
lab head
Balamurugan Packialakshmi
contact affiliation	University of Arkansas, Fayetteville, AR
contact email	balamurugan3@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD003335

PRIDE project URI

• PRIDE
  1. PXD003335
     1. Label: PRIDE project
     2. Name: Chicken plasma proteome LPS