PXD003326

PXD003326 is an original dataset announced via ProteomeXchange.

Title	Transgenic soybeans expressing human epidermal growth factor
Description	Each year in the United States, more than 530,000 babies are born prior to full 37 weeks of gestation. One of the major problems associated with prematurity is the development of a condition known as necrotizing enterocolitis (NEC). In 50% of infants with NEC a large amount of damaged and dead intestine must be surgically removed, often resulting in death or lifelong health problems. A growing body of experimental and clinical evidence supports a conclusion that deficient quantities of epidermal growth factor (EGF) leads to the development of NEC. The EGF peptide appears to be fundamental for normal intestinal development and repair. Soy-milk containing engineered EGF may be a suitable therapy if given to premature infants to prevent NEC. Human mature EGF is 6 kDa protein with three intramolecular disulfide bonds. To produce EGF a synthetic codon-optimized gene was transferred to soybean by biolistic transformation. The resulting transgenic lines were regenerated into somatic embryos that were screened by PCR and the production of EGF assayed by ELISA. Crude extracts of somatic embryos producing EGF were assessed for bioactivity by induction of phosphorylation of the EGF receptor in HeLa cells that showed soy-produced EGF exhibited the same activity as authentic EGF. T0 transgenic EGF seeds have showed positive ELISA for EGF demonstrating the feasibility to produce a therapeutic soy-milk to mitigate the development of NEC by premature infants. The next stage of this project will test the efficacy of Soy/EGF in an animal model for short bowel syndrome.
HostingRepository	PRIDE
AnnounceDate	2016-06-22
AnnouncementXML	Submission_2016-06-22_06:50:07.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003326
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ken Pendarvis
SpeciesList	scientific name: Glycine max (Soybean) (Glycine hispida); NCBI TaxID: 3847;
ModificationList	deamidated residue; monohydroxylated residue; dehydrated residue; acetylated residue; iodoacetamide derivatized residue; sulfated residue; deaminated residue; dihydroxylated residue
Instrument	LTQ Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-12-11 02:20:06	ID requested
⏵ 1	2016-06-22 06:50:08	announced

He Y, Schmidt MA, Erwin C, Guo J, Sun R, Pendarvis K, Warner BW, Herman EM, Transgenic Soybean Production of Bioactive Human Epidermal Growth Factor (EGF). PLoS One, 11(6):e0157034(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: LTQ Velos soybean Glycine max hispida epidermal growth factor transgenic

Monica Schnidt
contact affiliation	University of Arizona Department of Plant Sciences USA
contact email	monicaschmidt@email.arizona.edu
lab head
Ken Pendarvis
contact affiliation	Veterinary Science and Microbiology
contact email	jkpendarvis@email.arizona.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/06/PXD003326

PRIDE project URI

• PRIDE
  1. PXD003326
     1. Label: PRIDE project
     2. Name: Transgenic soybeans expressing human epidermal growth factor