PXD003321

PXD003321 is an original dataset announced via ProteomeXchange.

Title	Monitoring global protein thiol-oxidation and protein S-mycothiolation in Corynebacterium diphtheriae under hypochlorite stress
Description	Mycothiol (AcCys-GlcN-Ins, MSH) is the major thiol-redox buffer in Actinomycetes, including Mycobacterium and Corynebacterium species. Protein S-mycothiolation controls the activities of several redox enzymes that function in detoxification of ROS and methionine sulfoxides, including the thiol peroxidase Tpx, the mycothiol peroxidase Mpx and the methionine sulfoxide reductase MsrA. Here we investigated the level of protein S-mycothiolation in Corynebacterium diphtheriae DSM43989 under oxidative stress as well as its NaOCl stress response.
HostingRepository	PRIDE
AnnounceDate	2017-07-17
AnnouncementXML	Submission_2017-07-17_03:56:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Melanie Hillion
SpeciesList	scientific name: Corynebacterium diphtheriae; NCBI TaxID: 1717;
ModificationList	L-cystine (cross-link); cysteinylation (disulfide with free L-cysteine); Applied Biosystems cleavable ICAT(TM) heavy; N-ethylmaleimide derivatized cysteine; Applied Biosystems cleavable ICAT(TM) light; monohydroxylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-12-10 06:49:26	ID requested
⏵ 1	2017-07-17 03:56:41	announced

Hillion M, Imber M, Pedre B, Bernhardt J, Saleh M, Loi VV, Maa, ß S, Becher D, Astolfi Rosado L, Adrian L, Weise C, Hell R, Wirtz M, Messens J, Antelmann H, The glyceraldehyde-3-phosphate dehydrogenase GapDH of Corynebacterium diphtheriae is redox-controlled by protein S-mycothiolation under oxidative stress. Sci Rep, 7(1):5020(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: oxidative stress, bacteria, corynebacterium diphtheria, mycothiol, OxICAT, S-mycothiolation

Haike Antelmann
contact affiliation	Freie Universität Berlin Institut für Biologie-Mikrobiologie Königin-Luise-Straße 12-16 14195 Berlin Germany
contact email	haike.antelmann@fu-berlin.de
lab head
Melanie Hillion
contact affiliation	FU Berlin
contact email	melanie.hillion@fu-berlin.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/07/PXD003321

PRIDE project URI

• PRIDE
  1. PXD003321
     1. Label: PRIDE project
     2. Name: Monitoring global protein thiol-oxidation and protein S-mycothiolation in Corynebacterium diphtheriae under hypochlorite stress