PXD003319

PXD003319 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics uncovers novel factors involved in developmental differentiation of Trypanosoma brucei
Description	Developmental differentiation is a universal biological process that allows cells to adapt to different environments to perform specific functions. African trypanosomes progress through a tightly regulated life cycle in order to survive in different host environments when they shuttle between an insect vector and a vertebrate host. Transcriptomics has been useful to gain insight into RNA changes during stage transitions; however, RNA levels are only a moderate proxy for protein abundance in trypanosomes. We quantified 4270 protein groups during stage differentiation from the mammalian-infective to the insect form and provide classification for their expression profiles during development. Our label-free quantitative proteomics study revealed previously unknown components of the differentiation machinery that are involved in essential biological processes such as signaling, posttranslational protein modifications, trafficking and nuclear transport. Furthermore, guided by our proteomic survey, we identified the cause for the previously observed differentiation impairment in the histone methyltransferase DOT1B knock-out strain as it is required for accurate karyokinesis in the first cell division during differentiation. This epigenetic regulator is likely involved in essential chromatin restructuring during developmental differentiation, which might also be important for differentiation in higher eukaryotic cells. Our proteome dataset will serve as a resource for detailed investigations of cell differentiation to shed more light on the molecular mechanisms of this process in trypanosomes and other eukaryotes.
HostingRepository	PRIDE
AnnounceDate	2016-02-04
AnnouncementXML	Submission_2016-02-26_08:18:34.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	F Butter
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-12-10 02:17:36	ID requested
1	2016-02-04 09:21:57	announced
⏵ 2	2016-02-26 08:18:35	announced	Updated publication reference for PubMed record(s): 26910529.

Dejung M, Subota I, Bucerius F, Dindar G, Freiwald A, Engstler M, Boshart M, Butter F, Janzen CJ, Quantitative Proteomics Uncovers Novel Factors Involved in Developmental Differentiation of Trypanosoma brucei. PLoS Pathog, 12(2):e1005439(2016) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: trypanosomes

differentiation

Falk Butter
contact affiliation	Quantitative Proteomics Ackermannweg 4 55122 Mainz Germany
contact email	f.butter@imb-mainz.de
lab head
F Butter
contact affiliation	Dept. of Proteomics and Signal Transduction
contact email	f.butter@imb-mainz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/02/PXD003319

PRIDE project URI

• PRIDE
  1. PXD003319
     1. Label: PRIDE project
     2. Name: Quantitative proteomics uncovers novel factors involved in developmental differentiation of Trypanosoma brucei