PXD003318

PXD003318 is an original dataset announced via ProteomeXchange.

Title	Protein acetylation in young versus midlife Drosophila male heads
Description	Old age is associated with a progressive decline of mitochondrial function and changes in nuclear chromatin. However, little is known about how metabolic activity and epigenetic modifications change as organisms reach their midlife. Here, we assessed how protein acetylation changes during midlife in Drosophila melanogaster. Midlife flies show elevated acetyl-CoA levels and alterations in protein acetylation. Based on these observations, we decreased the activity of the acetyl-CoA-synthesizing enzyme ATP citrate lyase (ATPCL). We find that lower ATPCL activity alleviates the observed aging-associated changes and promote longevity. Our findings reveal a pathway that couples changes of intermediate metabolism during aging with the chromatin-mediated regulation of transcription and changes in the activity of associated enzymes that modulate organismal lifespan.
HostingRepository	PRIDE
AnnounceDate	2016-02-18
AnnouncementXML	Submission_2016-02-18_00:30:28.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignasi Forne
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	acetylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-12-10 01:35:03	ID requested
1	2016-01-22 08:38:20	announced
⏵ 2	2016-02-18 00:30:31	announced	Updated project metadata.

Peleg S, Feller C, Forne I, Schiller E, S, é, vin DC, Schauer T, Regnard C, Straub T, Prestel M, Klima C, Schmitt Nogueira M, Becker L, Klopstock T, Sauer U, Becker PB, Imhof A, Ladurner AG, Life span extension by targeting a link between metabolism and histone acetylation in Drosophila. EMBO Rep, 17(3):455-69(2016) [pubmed]

curator keyword: Biological

submitter keyword: Ageing, Protein acetylation, Drosophila

Axel Imhof
contact affiliation	Zentrallabor für Proteinanalytik LS Molekularbiologie BioMedical Center Ludwig-Maximilians-Universität Großhadernerstr. 9 82152 Planegg-Martinsried
contact email	imhof@lmu.de
lab head
Ignasi Forne
contact affiliation	Biomedical Center-LMU
contact email	ignasi.forne@lrz.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD003318

PRIDE project URI

• PRIDE
  1. PXD003318
     1. Label: PRIDE project
     2. Name: Protein acetylation in young versus midlife Drosophila male heads