⮝ Full datasets listing

PXD003316

PXD003316 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTumor matrix stiffness induces CCN1/CYR61 upregulation of N-Cadherin to promote cancer cell trans-endothelial migration and metastasis
DescriptionTumor progression is often accompanied with increased extracellular matrix stiffness, but how this affects endothelial cells (ECs) is largely unknown. We used mass spectrometry to analyse the proteomic changes of primary human ECs cultured on physiological or tumor stiffness and found that CCN1/CYR61 is highly induced by tumor stiffness. Knock out of Ccn1 in the vasculature of Ccn1loxP/loxP mice by administrating a soluble form of Cre shows that fewer of the treated mice harbour circulating tumor cells and lung metastases, without affecting primary tumor growth, using the B16F10 syngeneic mouse melanoma model This demonstrates that CCN1 loss in the host impairs cancer metastasis and we dissected the molecular mechanism in vitro. Stiffness-induced CCN1 acts via (integrin αvβ3), FAK, beta-catenin signalling to increase N-Cadherin expression in ECs, which, in turn, leads to an elevated adhesion of cancer cells to ECs via N-Cadherin homophilic interactions.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:27:02.057.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSara Zanivan
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-12-10 01:05:03ID requested
12017-07-07 06:54:00announced
22017-07-17 06:36:19announcedUpdated publication reference for PubMed record(s): 28694244.
32024-10-22 04:27:04announced2024-10-22: Updated project metadata.
Publication List
Reid SE, Kay EJ, Neilson LJ, Henze AT, Serneels J, McGhee EJ, Dhayade S, Nixon C, Mackey JB, Santi A, Swaminathan K, Athineos D, Papalazarou V, Patella F, Rom, á, n-Fern, á, ndez Á, ElMaghloob Y, Hernandez-Fernaud JR, Adams RH, Ismail S, Bryant DM, Salmeron-Sanchez M, Machesky LM, Carlin LM, Blyth K, Mazzone M, Zanivan S, Tumor matrix stiffness promotes metastatic cancer cell interaction with the endothelium. EMBO J, 36(16):2373-2389(2017) [pubmed]
10.15252/embj.201694912;
Keyword List
curator keyword: Biomedical
submitter keyword: stiffness
protome
SILAC
CCN1
Matrigel
HUVEC
tumour
vasculature
Contact List
Sara Zanivan
contact affiliationCRUK Beatson Institute
contact emails.zanivan@beatson.gla.ac.uk
lab head
Sara Zanivan
contact affiliationTumour Microenvironment & Proteomics
contact emails.zanivan@beatson.gla.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/07/PXD003316
PRIDE project URI
Repository Record List
[ + ]