PXD003311

PXD003311 is an original dataset announced via ProteomeXchange.

Title	CodY regulates the activity of the virulence quorum sensor PlcR
Description	In Gram-positive bacteria, cell-cell communication mainly relies on cytoplasmic sensors of the RNPP family. Activity of these regulators depends on their binding to secreted signaling peptides that are imported into the cell. These quorum sensing regulators control important biological functions in bacteria of the Bacillus cereus group, such as virulence and necrotrophism. The RNPP quorum sensor PlcR, in complex with its cognate signaling peptide PapR, is the main regulator of virulence in B. cereus and Bacillus thuringiensis (Bt). Recent reports have shown that the global stationary phase regulator CodY, involved in adaptation to nutritional limitation, is required for the expression of virulence genes belonging to the PlcR regulon. However, the mechanism underlying this regulation was not described. Using genetics and proteomics approaches, we showed that CodY regulates the expression of the virulence genes through the import of PapR. We report that CodY positively controls the production of the proteins that compose the oligopeptide permease OppABCDF, and of several other Opp-like proteins. It was previously shown that the pore components of this oligopeptide permease, OppBCDF, were required for the import of PapR. However, the role of OppA, the substrate-binding protein (SBP), was not investigated. Here, we demonstrated that OppA is not the only SBP involved in the recognition of PapR, and that several other OppA-like proteins can allow the import of this peptide. Altogether, these data complete our model of quorum sensing during the lifecycle of Bt and indicate that RNPPs integrate environmental conditions, as well as cell density, to coordinate the behavior of the bacteria throughout growth.
HostingRepository	PRIDE
AnnounceDate	2015-12-17
AnnouncementXML	Submission_2016-01-26_06:45:06.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	HENRY CELINE
SpeciesList	scientific name: Bacillus thuringiensis Bt407; NCBI TaxID: 527021;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-12-09 05:32:56	ID requested
1	2015-12-17 08:35:02	announced
⏵ 2	2016-01-26 06:45:08	announced	Updated publication reference for PubMed record(s): 26779156.

Slamti L, Lemy C, Henry C, Guillot A, Huillet E, Lereclus D, CodY Regulates the Activity of the Virulence Quorum Sensor PlcR by Controlling the Import of the Signaling Peptide PapR in Bacillus thuringiensis. Front Microbiol, 6():1501(2015) [pubmed]

curator keyword: Biological

submitter keyword: quorum-sensing, Bacillus, regulation, Cody, PlcR, signaling peptide, OPP, virulence regulon, Mass Spectrometry, Shot gun

Celine Henry
contact affiliation	INRA, UMR1319,MICALIS
contact email	celine.henry@jouy.inra.fr
lab head
HENRY CELINE
contact affiliation	INRA
contact email	celine.henry@jouy.inra.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003311
     1. Label: PRIDE project
     2. Name: CodY regulates the activity of the virulence quorum sensor PlcR