PXD003292

PXD003292 is an original dataset announced via ProteomeXchange.

Title	Chicken plasma proteome femoral head seperation
Description	Lameness due to femoral head separation (FHS) is a production and welfare issue in commercial poultry. FHS is an idiopathic disorder, which is attributed to a myriad of factors but in order to improve bone health, broiler breeders must be prognosed for disease susceptibility and selected against FHS using biomarkers. Proteins from plasma of blood, which can be obtained using minimally invasive methods represent an ideal, rich source of biomarkers which might be different in susceptible or affected birds. The peptide and proteins in plasma of healthy (HLTH) and affected birds (FHS) were compared using Matrix assisted laser desorption ionization mass spectrometry (MALDI-TOF-MS) and Liquid chromatography and tandem mass spectrometry (LC-MS/MS). The peptide profile of HLTH and FHS were compared using ClinPro tools and the differentially expressed peptides were isolated by Reverse phase liquid chromatography fractionation (C18-RP-HPLC) and identified using peptide mass fingerprinting. Peptides derived from fibrinogen precursor and fetuin were reduced in FHS birds. Based on the proteomic analysis, proteins such as Gallinacin 10, Apolipoprotein A-1 and Hemoglobin chains are elevated in FHS while Alpha 1-acid glycoprotein is reduced in FHS birds. Our study shows that bodyweight, lipid profile and the above mentioned proteins could be useful as a biomarker for improvement of bone health. These proteins indicate that blood lysis, antimicrobial defense and lipid disorder but lack of an inflammatory response might be consequential to FHS.
HostingRepository	PRIDE
AnnounceDate	2016-08-09
AnnouncementXML	Submission_2016-08-09_02:48:56.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003292
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Balamurugan Packialakshmi
SpeciesList	scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031;
ModificationList	monomethylated residue; dihydroxylated residue; sulfated residue; dehydrated residue; phosphorylated residue; trifluoroacetic acid adduct; monohydroxylated residue; acetylated residue; deaminated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Bruker Daltonics amaZon series

Revision	Datetime	Status	ChangeLog Entry
0	2015-12-07 04:54:36	ID requested
⏵ 1	2016-08-09 02:48:57	announced

Packialakshmi B, Liyanage R, Lay JO, Okimoto R, Rath NC, Proteomic Changes in the Plasma of Broiler Chickens with Femoral Head Necrosis. Biomark Insights, 11():55-62(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: chicken, femoral head necrosis, plasma, proteomics, biomarkers

Narayan C Rath
contact affiliation	Research Physiologist
contact email	narayan.rath@ars.usda.gov
lab head
Balamurugan Packialakshmi
contact affiliation	University of Arkansas, Fayetteville, AR
contact email	balamurugan3@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003292
     1. Label: PRIDE project
     2. Name: Chicken plasma proteome femoral head seperation