PXD003289

PXD003289 is an original dataset announced via ProteomeXchange.

Title	Dysregulation of Cell-Cell Interaction in Brain Arteriovenous Malformations: A Quantitative Proteomics Study
Description	Object. Detailed and exact mechanisms underlying brain arteriovenous malformations (bAVM) are still confusing in clinic. Understanding the quantitative changes of proteins and signaling pathways would provide useful information for clinicians to understand the formation and development of bAVM and therefore give proper individual treatment strategies. This study was performed to establish a huge human bAVM proteome database by tandem mass tag (TMT)-labeling technique and detect the alteration of proteins and pathways in the development of human bAVM. Methods. This study used quantitative proteomics to profile protein changes with the 6-plex TMT labeling in bAVM lesions. Integrated bioinformatics analysis were used to classify and identify the altered proteins and relating signaling pathways. Western blot analyses were used to identify the reliability of the proteomic data. Results. Our work established one of the largest human bAVM proteome databases to date. A total of 1264 proteins were identified, among which the expression of 316 proteins were changed with 249 proteins upregulated. Bioinformatics analysis revealed a close relevance between cell-cell interactions including focal adhesion, tight junction, gap junction and the development of bAVM. Conclusions. Cell-cell interactions, including focal adhesion, tight junction and gap junction played vital roles in the formation and development of bAVM. Understanding the molecular mechanism underlying bAVM is important for the development of future therapeutic approaches, thereafter making precise and individual treatment strategies in clinic.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:27:01.793.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wei Ge
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-12-07 02:48:12	ID requested
1	2017-01-19 23:53:14	announced
⏵ 2	2024-10-22 04:27:10	announced	2024-10-22: Updated project metadata.

10.1002/prca.201600093;

Wang X, Hao Q, Zhao Y, Guo Y, Ge W, Dysregulation of cell-cell interactions in brain arteriovenous malformations: A quantitative proteomic study. Proteomics Clin Appl, 11(5-6):(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: proteomics, quantitative,angiogenesis, brain arteriovenous malformations, cell-cell interaction

Wei Ge
contact affiliation	Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences
contact email	wei.ge@chem.ox.ac.uk
lab head
Wei Ge
contact affiliation	National Key Laboratory of Medical Molecular Biology & Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences
contact email	wei.ge@chem.ox.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003289
     1. Label: PRIDE project
     2. Name: Dysregulation of Cell-Cell Interaction in Brain Arteriovenous Malformations: A Quantitative Proteomics Study