PXD003285

PXD003285 is an original dataset announced via ProteomeXchange.

Title	Structural elucidation of a novel mechanism for bacteriophage-based inhibition of the RNA degradosome
Description	In all domains of life, the catalysed degradation of RNA facilitates rapid adaption to changing environmental conditions. We identified a virus-encoded protein that directly binds and inhibits the RNA degradation machinery of its bacterial host, allowing efficient accumulation of viral RNA in the infected cell. Encoded by the giant phage фKZ, KZ37/Dip associates with two RNA binding sites of the RNase E component of the Pseudomonas aeruginosa RNA degradosome. Thereby, KZ37/Dip competes with the binding of RNA substrates, resulting in effective inhibition of RNA degradation. The crystal structure of KZ37/Dip (2.2 Å) reveals an unprecedented fold for which there are no identified structural homologues. The protein forms a homo-dimer that resembles a partially opened scroll and binds RNase E through exposed acidic patches on its convex outer surface. Through the activity of KZ37/Dip, фKZ has evolved a unique mechanism to down regulate a key process of its host.
HostingRepository	PRIDE
AnnounceDate	2016-07-27
AnnouncementXML	Submission_2016-07-27_05:53:17.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003285
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jean-Paul Noben
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LCQ Classic

Revision	Datetime	Status	ChangeLog Entry
0	2015-12-07 01:58:52	ID requested
⏵ 1	2016-07-27 05:53:19	announced

Van den Bossche A, Hardwick SW, Ceyssens PJ, Hendrix H, Voet M, Dendooven T, Bandyra KJ, De Maeyer M, Aertsen A, Noben JP, Luisi BF, Lavigne R, Structural elucidation of a novel mechanism for the bacteriophage-based inhibition of the RNA degradosome. Elife, 5():(2016) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Phage, ΦKZ, RNA degradosome, protein-RNA interactions, protein-protein interactions, X-ray crystallography, Pseudomonas aeruginosa, Escherichia coli

Lavigne Rob
contact affiliation	Laboratory of Gene Technology Kasteelpark Arenberg 21 3001 Leuven (Heverlee) Belgium
contact email	rob.lavigne@kuleuven.be
lab head
Jean-Paul Noben
contact affiliation	FBI
contact email	jeanpaul.noben@uhasselt.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003285

PRIDE project URI

• PRIDE
  1. PXD003285
     1. Label: PRIDE project
     2. Name: Structural elucidation of a novel mechanism for bacteriophage-based inhibition of the RNA degradosome