PXD003270

PXD003270 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Site-specific O-Glycosylation Analysis of Human Blood Plasma Proteins
Description	Here, we report on the site-specific O-glycosylation analysis of human blood plasma glycoproteins. To this end pooled human blood plasma of healthy donors was digested non-specifically using Protein-ase K, followed by a precipitation step, as well as a glycopeptide enrichment and fractionation step via hydrophilic interaction liquid chromatography. Enriched glycopeptide fractions were subjected to mass spectrometric analysis using reversed-phase liquid chromatography coupled online to an ion trap mass spectrometer operated in positive-ion mode. Peptide identity and glycan composition were derived from low-energy collision-induced dissociation fragment spectra acquired in multistage mode. To pinpoint the O-glycosylation sites glyco�peptides were fragmented using electron transfer dissociation. Spectra were annotated by database searches as well as manually. Overall, 31 O-glycosylation sites and regions belonging to 22 proteins were identified. The majority of these proteins were acute-phase proteins. Strikingly, also 11 novel O-glycosylation sites and regions were identified. In total 23 O-glycosylation sites could be pinpointed. Interestingly, the use of Proteinase K proved to be particularly beneficial in this context. The identified O-glycan compositions most probably correspond to mono- and disialylated core-1 mucin-type O-glycans (T-antigen).
HostingRepository	MassIVE
AnnounceDate	2015-12-02
AnnouncementXML	Submission_2015-12-02_04:06:40.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marcus Hoffmann
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl; Deamidated; Oxidation
Instrument	amaZon ETD

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2015-12-02 04:06:05	ID requested
⏵ 1	2015-12-02 04:06:41	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Glycoproteomics, O-Glycosylation, Human Blood Plasma, Proteinase K

submitter keyword: Glycoproteomics, O-Glycosylation, Human Blood Plasma, Proteinase K

Contact List

Marcus Hoffmann, Kristina Marx, Udo Reichl, Manfred Wuhrer, Erdmann Rapp
lab head
Marcus Hoffmann
contact affiliation	Max Planck Institute for Dynamics of Complex Technical Systems
contact email	mhoffmann@mpi-magdeburg.mpg.de
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000079141