PXD003267

PXD003267 is an original dataset announced via ProteomeXchange.

Title	Changes in the plasma proteome of Manduca sexta larvae in relation to the transcriptome variations after an immune challenge: evidence for high molecular weight immune complex formation
Description	Manduca sexta is a lepidopteran model widely used to study insect physiological processes including innate immunity. In this study, we explored the proteomes of cell-free hemolymph from larvae injected with a sterile buffer (C for control) or a mixture of bacteria (I for induced). Of the 654 proteins identified, 70 showed 1.67 to >200 fold abundance increases after the immune challenge; 51 decreased to 0−60% of the control levels. While there was no strong parallel between plasma protein levels and their transcript levels in hemocytes or fat body, the mRNA level changes (i.e. I/C ratios of normalized read numbers) in the tissues concurred with their protein level changes (i.e. I/C ratios of normalized spectral counts) with correlation coefficients of 0.44 and 0.57, respectively. Better correlations support that fat body contributes a more significant portion of the plasma proteins involved in various aspects of innate immunity. Consistently, ratios of mRNA and protein levels were better correlated for immunity-related proteins than unrelated ones. There is a set of proteins whose apparent molecular masses differ considerably from the calculated Mr’s, suggestive of post-translational modifications. In addition, some low Mr proteins were detected in the range of 80 to >300 kDa on a reducing SDS-polyacrylamide gel, indicating the existence of high Mr covalent complexes. We identified 30 serine proteases and their homologs, eleven of which are known members of an extracellular immune signaling network. Along with our quantitative transcriptome data, the protein identification, inducibility, and association provide leads toward a focused exploration of humoral immunity in M. sexta.
HostingRepository	PRIDE
AnnounceDate	2016-02-01
AnnouncementXML	Submission_2016-02-01_08:56:20.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003267
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Xiaolong Cao
SpeciesList	scientific name: Manduca sexta (Tobacco hawkmoth) (Tobacco hornworm); NCBI TaxID: 7130;
ModificationList	Oxidation; Gln->pyro-Glu; Propionamide; Formyl; Acetyl; Carbamidomethyl
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2015-12-02 02:37:44	ID requested
⏵ 1	2016-02-01 08:56:22	announced

He Y, Cao X, Zhang S, Rogers J, Hartson S, Jiang H, Changes in the Plasma Proteome of Manduca sexta Larvae in Relation to the Transcriptome Variations after an Immune Challenge: Evidence for High Molecular Weight Immune Complex Formation. Mol Cell Proteomics, 15(4):1176-87(2016) [pubmed]

curator keyword: Biological

submitter keyword: insect immunity, hemolymph proteins, LC-MS/MS, spectral counting, tobacco hornworm, protein processing, phenoloxidase activation

Haobo Jiang
contact affiliation	Department of Entomology and Plant Pathology, 127 Noble Research Center, Oklahoma State University, Stillwater, Oklahoma, USA, 74078
contact email	haobo.jiang@okstate.edu
lab head
Xiaolong Cao
contact affiliation	Oklahoma State University
contact email	xiaolong.cao@okstate.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003267
     1. Label: PRIDE project
     2. Name: Changes in the plasma proteome of Manduca sexta larvae in relation to the transcriptome variations after an immune challenge: evidence for high molecular weight immune complex formation