PXD003260

PXD003260 is an original dataset announced via ProteomeXchange.

Title	Proteomics assisted studies of the host nuclear proteome identifies candidate regulators of immunity modified during Phytophthora capsici-tomato interactions
Description	In this study we have combined nuclei enrichment with quantitative proteomics to study tomato nuclear proteome dynamics during early infection with the devastating broad host-range oomycete pathogen Phytophthora capsici. Using this method we have identified hundreds of host nuclear proteins that show specific changes in abundance during P. capsici infection including proteins implicated in nucleic acid binding, oxidoreductase activity, helicase activity, RNA binding and transcription regulator activity. A number of these proteins have known links to plant immunity or defence signalling. We have confirmed the nuclear localisation of a selection of proteins which show P. capsici infection dependant changes in abundance and have examined the impact of their expression on resistance. As direct proof of biological relevance we demonstrate that in planta overexpression of an AT-hook family protein which decreased in abundance during infection, causes enhanced resistance to P. capsici. We conclude that organelle enrichment combined with quantitative proteomics represents a valuable strategy, suited to interrogate dynamic proteomes during stress, and allows the identification of promising targets to bolster immunity in crops.
HostingRepository	PRIDE
AnnounceDate	2017-04-18
AnnouncementXML	Submission_2017-04-18_09:10:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrew Howden
SpeciesList	scientific name: Solanum lycopersicum; NCBI TaxID: 4081;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-12-01 01:36:43	ID requested
⏵ 1	2017-04-18 09:10:42	announced

Howden AJM, Stam R, Martinez Heredia V, Motion GB, Ten Have S, Hodge K, Marques Monteiro Amaro TM, Huitema E, Quantitative analysis of the tomato nuclear proteome during Phytophthora capsici infection unveils regulators of immunity. New Phytol, 215(1):309-322(2017) [pubmed]

curator keyword: Biological

submitter keyword: nucleus, quantitative proteomics, Phytophthora, plant-microbe interactions, tomato, immunity

Dr Edgar Huitema
contact affiliation	Division of Plant Sciences, University of Dundee at JHI, Scotland, UK
contact email	e.huitema@dundee.ac.uk
lab head
Andrew Howden
contact affiliation	University of Dundee
contact email	a.howden@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/04/PXD003260

PRIDE project URI

• PRIDE
  1. PXD003260
     1. Label: PRIDE project
     2. Name: Proteomics assisted studies of the host nuclear proteome identifies candidate regulators of immunity modified during Phytophthora capsici-tomato interactions