PXD003257

PXD003257 is an original dataset announced via ProteomeXchange.

Title	Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes
Description	The extracellular vesicles (EVs) have become the focus of rising interest, because of their numerous functions in physiology and pathology. Cells release heterogeneous vesicles of different sizes and intracellular origins, including small EVs formed inside endosomal compartments (i.e. exosomes) and EVs of various sizes budding from the plasma membrane. Specific markers for the analysis and isolation of different EV populations are missing, imposing important limitations to understanding EV functions. Here, EVs from human dendritic cells were first separated by their sedimentation speed and then either by their behavior upon upward floatation into iodixanol gradients, or by immuno-isolation. Extensive quantitative proteomic analysis allowing comparison of the isolated populations showed that several classically used exosome markers, like major histocompatibility complex, flotillin and heat shock 70 kDa proteins, are similarly present in all EVs. We identified proteins specifically enriched in small EVs, and define a new set of five protein categories displaying different relative abundance in distinct EV populations. We demonstrate the presence of exosomal and non-exosomal subpopulation within small EVs, and propose their differential separation by immuno-isolation using either CD63, CD81 or CD9. Our work thus provides new guidelines to define subtypes of EVs for future functional studies.
HostingRepository	PRIDE
AnnounceDate	2016-02-11
AnnouncementXML	Submission_2016-02-11_01:09:21.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guillaume Arras
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2015-11-30 07:44:31	ID requested
⏵ 1	2016-02-11 01:09:22	announced

Kowal J, Arras G, Colombo M, Jouve M, Morath JP, Primdal-Bengtson B, Dingli F, Loew D, Tkach M, Th, é, ry C, Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes. Proc Natl Acad Sci U S A, 113(8):E968-77(2016) [pubmed]

curator keyword: Biological

submitter keyword: exosomes, microvesicles, ectosomes, dendritic cells, extracellular vesicles

Damarys Loew
contact affiliation	Laboratoire de Spectrométrie de Masse Protéomique (LSMP) Institut Curie
contact email	damarys.loew@curie.fr
lab head
Guillaume Arras
contact affiliation	Institut Curie
contact email	guillaume.arras@curie.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/02/PXD003257

PRIDE project URI

• PRIDE
  1. PXD003257
     1. Label: PRIDE project
     2. Name: Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes