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PXD003251

PXD003251 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComparative proteomics identifies host immune system proteins affected by infection with Mycobacterium bovis
DescriptionMycobacteria of the Mycobacterium tuberculosis complex (MTBC) greatly impact on human and animal health worldwide. Mycobacterial life cycle is complex and the mechanisms resulting in pathogen infection and survival in host cells are not fully understood. Eurasian wild boar (Sus scrofa) are natural reservoir hosts for MTBC and a model for mycobacterial infection and tuberculosis (TB). In the wild boar TB model, mycobacterial infection affects the expression of innate and adaptive immune response genes in mandibular lymph nodes and oropharyngeal tonsils and biomarkers have been proposed as correlates with resistance to natural infection. However, the mechanisms used by mycobacteria to manipulate host immune response are not fully characterized. Our hypothesis is that the immune system proteins under-represented in infected animals when compared to uninfected controls are used by mycobacteria to guarantee pathogen infection and transmission. To address this hypothesis, a comparative proteomics approach was used to compare host response between uninfected (TB-) and M. bovis-infected young (TB+) and adult animals with different infection status [TB lesions localized in the head (TB+) or affecting multiple organs (TB++)]. The results identified host immune system proteins that play an important role in host response to mycobacteria. Calcium binding protein A9, Heme peroxidase, Lactotransferrin, Cathelicidin and Peptidoglycan-recognition protein were under-represented in TB+ animals when compared to uninfected TB- controls but protein levels increased as infection progressed in TB++ animals when compared to TB- and/or TB+ adult wild boar. MHCI was the only protein over-represented in TB+ adult wild boar when compared to uninfected TB- controls. The results reported here suggested that M. bovis manipulates host immune response by reducing the production of immune system proteins. However, as infection progresses, wild boar immune response recover to limit pathogen multiplication and promote survival that also facilitates pathogen transmission.
HostingRepositoryPRIDE
AnnounceDate2016-03-10
AnnouncementXMLSubmission_2016-04-01_04:29:38.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD003251
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMargarita Villar
SpeciesList scientific name: Sus scrofa (Pig); NCBI TaxID: 9823;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-11-30 02:06:14ID requested
12016-03-10 03:12:50announced
22016-04-01 04:29:39announcedUpdated publication reference for PubMed record(s): 27027307.
Publication List
L, ó, pez V, Villar M, Queir, ó, s J, Vicente J, Mateos-Hern, á, ndez L, D, í, ez-Delgado I, Contreras M, Alves PC, Alberdi P, Gort, á, zar C, de la Fuente J, Comparative Proteomics Identifies Host Immune System Proteins Affected by Infection with Mycobacterium bovis. PLoS Negl Trop Dis, 10(3):e0004541(2016) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Wild boar, immune system, LC-MS/MS
Contact List
Margarita Villar
contact affiliationProteomics Lab. SaBio research group. Instituto de Investigación en Recursos Cinegéticos, IREC (CSIC-UCLM.JCCM)
contact emailmargaritam.villar@uclm.es
lab head
Margarita Villar
contact affiliationSaBio (Sanidad y Biotecnología) Research Group. Instituto de Investigación en Recursos Cinegéticos, IREC (CSIC-UCLM-JCCM)
contact emailmargaritam.villar@uclm.es
dataset submitter
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Dataset FTP location
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