<<< Full experiment listing

PXD003249

PXD003249 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleOuter membrane vesicle and outer membrane of Haemophilus influenzae Rd KW20, Rd ∆yrbE and Rd ∆vacJ
DescriptionBacterial outer membrane vesicles (OMVs) have important biological roles in pathogenesis and intercellular interactions, but a general mechanism of OMV formation is lacking. Here we show that the VacJ/Yrb ABC transport system, a proposed phospholipid (PL) transporter, is involved in OMV formation. Deletion or repression of VacJ/Yrb increases OMV production in two distantly related Gram-negative bacteria, Haemophilus influenzae and Vibrio cholerae. Within our studies we also analyzed the proteome of the OMV and outer membrane (OM) and found no massive alteration in Haemophilus influenzae Rd KW20, Rd ∆yrbE and Rd ∆vacJ. Lipidome analyses demonstrate that OMVs from VacJ/Yrb-defective mutants in H. influenzae are enriched in PLs and certain fatty acids. Furthermore, we demonstrate that OMV production and regulation of the VacJ/Yrb ABC transport system respond to iron starvation. Our results suggest a new general mechanism of OMV biogenesis based on PL accumulation in the outer leaflet of the outer membrane. This mechanism is highly conserved among Gram-negative bacteria, provides a means for regulation, can account for OMV formation under all growth conditions, and might have important pathophysiological roles in vivo.
HostingRepositoryPRIDE
AnnounceDate2016-01-27
AnnouncementXMLSubmission_2016-01-27_01:17:55.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterStefan Schild
SpeciesList scientific name: Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd); NCBI TaxID: 71421;
ModificationListiodoacetamide derivatized residue
InstrumentQ-Tof ultima
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-11-30 00:46:50ID requested
12016-01-27 01:17:57announced
Publication List
Keyword List
curator keyword: Biomedical
submitter keyword: OMV, OM, Haemophilus influenzae, Mla Pathway, Retrograde lipid trafficking system
Contact List
Stefan Schild
contact affiliationUniversity of Graz, Austria
contact emailstefan.schild@uni-graz.at
lab head
Stefan Schild
contact affiliationUniversity of Graz
contact emailstefan.schild@uni-graz.at
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD003249
PRIDE project URI
Repository Record List
[ + ]