PXD003249 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Outer membrane vesicle and outer membrane of Haemophilus influenzae Rd KW20, Rd ∆yrbE and Rd ∆vacJ |
Description | Bacterial outer membrane vesicles (OMVs) have important biological roles in pathogenesis and intercellular interactions, but a general mechanism of OMV formation is lacking. Here we show that the VacJ/Yrb ABC transport system, a proposed phospholipid (PL) transporter, is involved in OMV formation. Deletion or repression of VacJ/Yrb increases OMV production in two distantly related Gram-negative bacteria, Haemophilus influenzae and Vibrio cholerae. Within our studies we also analyzed the proteome of the OMV and outer membrane (OM) and found no massive alteration in Haemophilus influenzae Rd KW20, Rd ∆yrbE and Rd ∆vacJ. Lipidome analyses demonstrate that OMVs from VacJ/Yrb-defective mutants in H. influenzae are enriched in PLs and certain fatty acids. Furthermore, we demonstrate that OMV production and regulation of the VacJ/Yrb ABC transport system respond to iron starvation. Our results suggest a new general mechanism of OMV biogenesis based on PL accumulation in the outer leaflet of the outer membrane. This mechanism is highly conserved among Gram-negative bacteria, provides a means for regulation, can account for OMV formation under all growth conditions, and might have important pathophysiological roles in vivo. |
HostingRepository | PRIDE |
AnnounceDate | 2016-01-27 |
AnnouncementXML | Submission_2016-01-27_01:17:55.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Stefan Schild |
SpeciesList | scientific name: Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd); NCBI TaxID: 71421; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q-Tof ultima |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-11-30 00:46:50 | ID requested | |
⏵ 1 | 2016-01-27 01:17:57 | announced | |
Publication List
Keyword List
curator keyword: Biomedical |
submitter keyword: OMV, OM, Haemophilus influenzae, Mla Pathway, Retrograde lipid trafficking system |
Contact List
Stefan Schild |
contact affiliation | University of Graz, Austria |
contact email | stefan.schild@uni-graz.at |
lab head | |
Stefan Schild |
contact affiliation | University of Graz |
contact email | stefan.schild@uni-graz.at |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003249
- Label: PRIDE project
- Name: Outer membrane vesicle and outer membrane of Haemophilus influenzae Rd KW20, Rd ∆yrbE and Rd ∆vacJ