PXD003239

PXD003239 is an original dataset announced via ProteomeXchange.

Title	Global Subcellular Characterization of Protein Degradation Using Quantitative Proteomics
Description	Protein degradation provides an important regulatory mechanism used to control cell cycle progression and many other cellular pathways. To comprehensively analyze the spatial control of protein degradation in U2OS osteosarcoma cells, we have combined drug treatment and SILAC-based quantitative mass spectrometry with subcellular and protein fractionation. The resulting data set analyzed more than 74,000 peptides, corresponding to ∼5000 proteins, from nuclear, cytosolic, membrane, and cytoskeletal compartments. These data identified rapidly degraded proteasome targets, such as PRR11 and highlighted a feedback mechanism resulting in translation inhibition, induced by blocking the proteasome. We show this is mediated by activation of the unfolded protein response. We observed compartment-specific differences in protein degradation, including proteins that would not have been characterized as rapidly degraded through analysis of whole cell lysates. Bioinformatic analysis of the entire data set is presented in the Encyclopedia of Proteome Dynamics, a web-based resource, with proteins annotated for stability and subcellular distribution.
HostingRepository	PRIDE
AnnounceDate	2015-11-30
AnnouncementXML	Submission_2015-11-30_04:31:33.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vackar Afzal
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	deamidated residue; monohydroxylated residue; acetylated residue; 2-pyrrolidone-5-carboxylic acid (Gln)
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-11-26 01:43:46	ID requested
⏵ 1	2015-11-30 04:31:34	announced

Larance M, Ahmad Y, Kirkwood KJ, Ly T, Lamond AI, Global subcellular characterization of protein degradation using quantitative proteomics. Mol Cell Proteomics, 12(3):638-50(2013) [pubmed]

curator keyword: Biological

submitter keyword: U2OS, PRR11, nuclear, cytosolic, membrane, cytoskeletal,osteosarcoma cells

Angus Lamond
contact affiliation	Principle Investigator
contact email	ailamond@dundee.ac.uk
lab head
Vackar Afzal
contact affiliation	School of Life Sciences
contact email	v.z.afzal@dundee.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003239
     1. Label: PRIDE project
     2. Name: Global Subcellular Characterization of Protein Degradation Using Quantitative Proteomics