PXD003231

PXD003231 is an original dataset announced via ProteomeXchange.

Title	Bacterial antisense RNAs are mainly the product of transcriptional noise
Description	Cis-encoded antisense RNAs (asRNAs) are widespread along bacterial transcriptomes. However, the role of the vast majority of these RNAs remains unknown, and there is an ongoing discussion as to what extent these transcripts are the result of transcriptional noise. We show, by comparative transcriptomics of 20 bacterial species and one chloroplast, that the number of asRNAs is exponentially dependent on the genomic AT content, and that expression of asRNA at low levels exerts little impact in terms of energy consumption. A transcription model simulating mRNA and asRNA production indicates that the asRNA regulatory effect is only observed above certain expression thresholds, substantially higher than physiological transcript levels. These predictions were verified experimentally by overexpressing 9 different asRNAs in M. pneumoniae. Our results suggest that most of the antisense transcripts found in bacteria are the consequence of transcriptional noise, arising at spurious promoters throughout the genome.
HostingRepository	PRIDE
AnnounceDate	2018-10-25
AnnouncementXML	Submission_2018-10-25_06:35:59.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Veronica Llorens-Rico
SpeciesList	scientific name: Mycoplasma pneumoniae (strain ATCC 29342 / M129); NCBI TaxID: 272634;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-11-25 01:24:41	ID requested
⏵ 1	2018-10-25 06:36:00	announced

Llor, é, ns-Rico V, Cano J, Kamminga T, Gil R, Latorre A, Chen WH, Bork P, Glass JI, Serrano L, Lluch-Senar M, Bacterial antisense RNAs are mainly the product of transcriptional noise. Sci Adv, 2(3):e1501363(2016) [pubmed]

curator keyword: Biological

submitter keyword: asRNAs, non-coding RNAs, bacteria, sRNAs

Luis Serrano
contact affiliation	Centre for Genomic Regulation, CRG
contact email	luis.serrano@crg.eu
lab head
Veronica Llorens-Rico
contact affiliation	Centre for Genomic Regulation, CRG
contact email	veronica.llorens@crg.eu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003231
     1. Label: PRIDE project
     2. Name: Bacterial antisense RNAs are mainly the product of transcriptional noise