PXD003229

PXD003229 is an original dataset announced via ProteomeXchange.

Title	Comprehensive Proteomic Analysis of Nitrogen Starved Mycobacterium smegmatis Δpup Reveals the Impact of Pupylation on Nitrogen Stress Response
Description	Mycobacterium smegmatis is a soil bacterium exposed to continuously environmental changes of nitrogen availability. As member of Actinobacteria, it possesses an ubiquitin-like post-translational modification pathway called pupylation. The pathway starts when a small protein called Pup is attached to a lysine of a specific cellular target. Then the Pup-modified protein is degraded by the proteasome, as it was shown that Pup acts as degradation signal. Recent studies showed the role of pupylation in Mycobacterium smegmatis for survival under nitrogen starvation by supplying recycled amino acids. The present study is an investigation of the influence of Mycobacterium smegmatis Pup protein on the whole proteome in absence of nitrogen sources. Therefore a pup deletion mutant was generated. Applying stable isotope dimethyl labelling, low impact of the pupylation on proteome was revealed immediately after exposure to growth medium lacking nitrogen. In contrast, post 24 h of nitrogen starvation, Msm pup deletion strain showed several proteins with significant changes in abundance. Noteworthy, key proteins involved in nitrogen assimilation were significantly affected in Msm Δpup. Furthermore, we label-free quantified pupylated proteins of nitrogen starved Msm for a more extensive understanding of pupylation role in surviving and overcoming the lack of nitrogen.
HostingRepository	PRIDE
AnnounceDate	2016-08-11
AnnouncementXML	Submission_2016-08-11_02:45:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Giuseppina Fascellaro
SpeciesList	scientific name: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155); NCBI TaxID: 246196;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap XL; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2015-11-24 23:21:16	ID requested
⏵ 1	2016-08-11 02:45:52	announced

Fascellaro G, Petrera A, Lai ZW, Nanni P, Grossmann J, Burger S, Biniossek ML, Gomez-Auli A, Schilling O, Imkamp F, pup Reveals the Impact of Pupylation on Nitrogen Stress Response. J Proteome Res, 15(8):2812-25(2016) [pubmed]

curator keyword: Biological

submitter keyword: pupylation, Mycobacterium smegmatis, nitrogen starvation, quantitative proteomics

Frank Imkamp
contact affiliation	University of Zurich, Institute of Medical Microbiology, Zurich, Switzerland
contact email	imkamp@imm.uzh.ch
lab head
Giuseppina Fascellaro
contact affiliation	University of Zurich
contact email	gfascellaro@imm.uzh.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003229
     1. Label: PRIDE project
     2. Name: Comprehensive Proteomic Analysis of Nitrogen Starved Mycobacterium smegmatis Δpup Reveals the Impact of Pupylation on Nitrogen Stress Response