PXD003218

PXD003218 is an original dataset announced via ProteomeXchange.

Title	Human serum from patients at multiple time points after ischemic stroke
Description	Application of acute therapies such as thrombolysis for ischemic stroke (IS) is constrained because of diagnostic uncertainty and the dynamic nature of stroke biology. To investigate changes in blood proteins after stroke and as a result of thrombolysis treatment we performed label-free quantitative proteomics on serum samples using high resolution mass spectrometry and long HPLC gradient (5 hours) combined with a 50cm column to optimize the peptide separation. We identified (FDR: 1%) and quantified a total of 574 protein groups from a total of 92 samples from 30 patients. Ten patients were treated by thrombolysis as part of a randomised placebo controlled trial and up to five samples were collected from each individual at different time points after stroke. We identified 26 proteins differently expressed by treatment group (FDR: 5%) and significant changes of expression over time for 23 proteins (FDR: 10%). Molecules such as fibrinogen and CRP showed expression profiles with a high potential clinical utility in the acute stroke setting. Protein expression profiles vary acutely in the blood after stroke and have the potential to allow the construction of a stroke clock and to have an impact on IS treatment decision making.
HostingRepository	PRIDE
AnnounceDate	2018-10-25
AnnouncementXML	Submission_2018-10-25_05:22:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ira Cooke
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2015-11-20 00:55:25	ID requested
⏵ 1	2018-10-25 05:22:19	announced

Dagonnier M, Cooke IR, Faou P, Sidon TK, Dewey HM, Donnan GA, Howells DW, Discovery and Longitudinal Evaluation of Candidate Biomarkers for Ischaemic Stroke by Mass Spectrometry-Based Proteomics. Biomark Insights, 12():1177271917749216(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: Human, ischemic stroke, serum

David William Howells
contact affiliation	School of Medicine, Faculty of Health, University of Tasmania, Hobart, Australia
contact email	david.howells@utas.edu.au
lab head
Ira Cooke
contact affiliation	La Trobe University
contact email	i.cooke@latrobe.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD003218

PRIDE project URI

• PRIDE
  1. PXD003218
     1. Label: PRIDE project
     2. Name: Human serum from patients at multiple time points after ischemic stroke