PXD003212

PXD003212 is an original dataset announced via ProteomeXchange.

Title	The Trichomonas PACK proteome
Description	Certain protist lineages bear cytoskeletal structures that are germane to them and define their individual group. Trichomonadida are excavate parasites united by a unique cytoskeletal framework, which includes tubulin-based structures such as the pelta and axostyle, but also other filaments such as the striated costa whose protein composition remains unknown. We determined the proteome of the detergent-resistant cytoskeleton of Tetratrichomonas gallinarum. 203 proteins with homology to Trichomonas vaginalis were identified, which contain significantly more long coiled-coil regions than control protein sets. Five candidates were shown to associate with previously described cytoskeletal structures including the costa and the expression of a single T. vaginalis protein in T. gallinarum induced the formation of accumulated, striated filaments. Our data suggests that filament-forming proteins of protists other than actin and tubulin share common structural properties with metazoan intermediate filament proteins, while not being homologous. These filament-forming proteins might have evolved many times independently in eukaryotes, or simultaneously in a common ancestor but with different evolutionary trajectories downstream in different phyla. The broad variety of filament-forming proteins uncovered, and with no homologs outside of the Trichomonadida, once more highlights the diverse nature of eukaryotic proteins with the ability to form unique cytoskeletal filaments.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_01:53:43.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003212
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Gereon Poschmann
SpeciesList	scientific name: Tetratrichomonas gallinarum; NCBI TaxID: 5730;
ModificationList	Oxidation; Acetyl; Carbamidomethyl
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2015-11-19 01:03:09	ID requested
1	2016-10-10 07:10:14	announced
2	2016-10-18 02:56:02	announced	Updated publication reference for PubMed record(s): 27744090.
3	2016-11-15 05:01:59	announced	Updated project metadata.
⏵ 4	2017-10-24 01:53:44	announced	Updated project metadata.

Preisner H, Karin EL, Poschmann G, St, ü, hler K, Pupko T, Gould SB, The Cytoskeleton of Parabasalian Parasites Comprises Proteins that Share Properties Common to Intermediate Filament Proteins. Protist, 167(6):526-543(2016) [pubmed]

curator keyword: Biological

submitter keyword: LC-MS, Tetratrichomonas gallinarum, evolution, cytoskeleton

Gereon Poschmann
contact affiliation	Molecular Proteomics Laboratory Heinrich Heine Universität Universitätsstr. 1 40225 Düsseldorf
contact email	gereon.poschmann@hhu.de
lab head
Gereon Poschmann
contact affiliation	Heinrich Heine Universität Düsseldorf
contact email	gereon.poschmann@rub.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD003212

PRIDE project URI

• PRIDE
  1. PXD003212
     1. Label: PRIDE project
     2. Name: The Trichomonas PACK proteome