PXD003210

PXD003210 is an original dataset announced via ProteomeXchange.

Title	REST-interactome
Description	Namgyu Lee has participated in the experimental part of this project and she is a co-first author of the under-going manuscript. RE-1 silencing transcription factor (REST) is a transcriptional repressor with a role in regulating gene expression through binding to repressor element 1. We identified REST/NRSF-interacting proteins by proteomics-based analyses using the complementary mass spectrometry approach. Our interactome revealed 204 REST-interacting proteins. Among those proteins, nuclear proteins were mostly enriched reflecting nuclear localization of REST. The interaction networks of interactome indicated biological processes associated with mRNA processing, chromatin organization and transcription. Interactions of ALYREF, HnRNP M, HnRNP Q, NPM1, NCL, PARP1, HDAC5, TRIM28 and HMGA1 with REST were confirmed by co-immunoprecipitation. Using public microarray dataset, a highly significant overlaps were observed in differentially expressed transcripts following knockdown of REST and interacting proteins such as HDAC5, HMGA1 and TRIM28, suggesting that the REST might cross-talk with those transcription regulators to regulate transcription of shared target genes. Our interactomic study of REST implies novel cross-talks with transcription regulators by its associations with interacting proteins.
HostingRepository	PRIDE
AnnounceDate	2018-10-25
AnnouncementXML	Submission_2018-10-25_04:32:39.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dae-Kyum Kim
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-11-18 06:27:46	ID requested
⏵ 1	2018-10-25 04:32:40	announced

Lee N, Park SJ, Haddad G, Kim DK, Park SM, Park SK, Choi KY, Interactomic analysis of REST/NRSF and implications of its functional links with the transcription suppressor TRIM28 during neuronal differentiation. Sci Rep, 6():39049(2016) [pubmed]

curator keyword: Biological

submitter keyword: Interaction profiling, Proteomics, Cell biology, LC-MS/MS, Transcription factor

Kwan Yong Choi
contact affiliation	Department of Life Science, Pohang University of Science and Technology, Pohang, Gyeongbuk, Korea Department of Integrative Biosciences & Biotechnology, Pohang, Gyeongbuk, Republic of Korea
contact email	kchoi@postech.ac.kr
lab head
Dae-Kyum Kim
contact affiliation	Department of Life Science
contact email	sses162@postech.ac.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD003210

PRIDE project URI

• PRIDE
  1. PXD003210
     1. Label: PRIDE project
     2. Name: REST-interactome