PXD003207

PXD003207 is an original dataset announced via ProteomeXchange.

Title	Sepiadarium austrinum Slime MSMS
Description	Sepiadarium austrinum, the southern bottletail squid, is a small squid that inhabits soft sediments along Australia’s south-east coast. When provoked this squid rapidly secretes large volumes of slime as a defense mechanism. Behavioral observations suggest that this slime may be toxic to crabs but its composition remains unknown. A reference transcriptome for S. austrinum using give tissues including slime was assembled and a database of 40,444 predicted proteins was created using the Trinotate annotation software with an addition 53 short proteins identified exclusively by proteogenomics. This database was used to identify 1736 proteins within the slime using bottom-up (shotgun) proteomics. The mechanism of slime secretion is likely to involve ejection of cell content with the presence of proteins involved in fundamental intracellular functions such as DNA binding and protein folding being highly abundant, along with few slime proteins containing a signal peptide. Putative toxic proteins were identified within the slime based on characteristics of known toxins, namely cysteine richness, short length, the existence of a signal peptide and homology to known toxins. Our study also adds a crucial layer of evidence for toxicity of these proteins by using direct proteomic measurement within toxic secretions (slime) rather than their parent glands. To our knowledge this is the first such study in cephalopods. Quantitative proteomics has allowed us to highlight two putative toxins within the top ten most abundant slime proteins. The slime secreted by S. austrinum may act as a carrier medium for these putative toxins and this may represent a novel toxin delivery mechanism for cephalopods.
HostingRepository	PRIDE
AnnounceDate	2016-08-08
AnnouncementXML	Submission_2016-08-08_07:15:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nikeisha Caruana
SpeciesList	scientific name: Sepiadarium austrinum; NCBI TaxID: 279093;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2015-11-18 03:07:47	ID requested
⏵ 1	2016-08-08 07:15:45	announced

Caruana NJ, Cooke IR, Faou P, Finn J, Hall NE, Norman M, Pineda SS, Strugnell JM, A combined proteomic and transcriptomic analysis of slime secreted by the southern bottletail squid, Sepiadarium austrinum (Cephalopoda). J Proteomics, 148():170-82(2016) [pubmed]

curator keyword: Biological

submitter keyword: Orbitrap, Squid, Cephalopod, Slime

Jan Marie Strugnell
contact affiliation	Department of Ecology, Environment and Evolution, School of Life Sciences, La Trobe University
contact email	J.Strugnell@latrobe.edu.au
lab head
Nikeisha Caruana
contact affiliation	La Trobe University
contact email	njcaruana@students.latrobe.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD003207

PRIDE project URI

• PRIDE
  1. PXD003207
     1. Label: PRIDE project
     2. Name: Sepiadarium austrinum Slime MSMS