PXD003202

PXD003202 is an original dataset announced via ProteomeXchange.

Title	Symbiosis induces widespread changes in the proteome of the model cnidarian Aiptasia
Description	Coral reef ecosystems are metabolically founded on the mutualism between corals and photosynthetic dinoflagellates of the genus Symbiodinium. The glass anemone Aiptasia sp. has become a tractable model for this symbiosis. We utilized label-free liquid chromatography electrospray-ionization tandem mass spectrometry to analyze the effects of symbiosis on the proteomes of symbiotic and aposymbiotic Aiptasia. We quantified more than 3,300 proteins in 1,578 protein clusters, with 81 protein clusters showing significantly different expression. Symbiotic anemones showed higher expression of proteins involved in lipid storage and transport, nitrogen transport and cycling, intracellular trafficking, endocytosis and inorganic carbon transport. These changes reflect shifts in host metabolism and energy reserves due to increased organic and inorganic nutritional exchange with the symbionts. Aposymbiotic anemones exhibited increased expression of multiple systems responsible for mediating reactive oxygen stress, suggesting that the host derives direct or indirect protection from oxidative stress while in symbiosis. Aposymbiotic anemones also increased their expression of an array of proteases and chitinases, indicating a metabolic shift from autotrophy to heterotrophy. These results provide a comprehensive Aiptasia proteome with more direct relative quantification of protein abundance than transcriptomic methods, allowing more powerful studies of coral physiology and ecosystem function.
HostingRepository	PRIDE
AnnounceDate	2017-02-22
AnnouncementXML	Submission_2017-02-22_06:00:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Clinton Oakley
SpeciesList	scientific name: Aiptasia pulchella (Tropical sea anemone); NCBI TaxID: 12924;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-11-17 06:04:04	ID requested
⏵ 1	2017-02-22 06:00:53	announced

Oakley CA, Ameismeier MF, Peng L, Weis VM, Grossman AR, Davy SK, Symbiosis induces widespread changes in the proteome of the model cnidarian Aiptasia. Cell Microbiol, 18(7):1009-23(2016) [pubmed]

curator keyword: Biological

submitter keyword: Aiptasia, cnidarian, symbiosis, label-free, coral, model organism

Simon Davy
contact affiliation	School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand
contact email	simon.davy@vuw.ac.nz
lab head
Clinton Oakley
contact affiliation	Victoria University of Wellington
contact email	clint.oakley@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003202
     1. Label: PRIDE project
     2. Name: Symbiosis induces widespread changes in the proteome of the model cnidarian Aiptasia