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PXD003201

PXD003201 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleImmunoproteomic analysis of antibody responses to extracellular proteins of Candida albicans in serum of candidemia patients revealed glycosylation effects on protein antigen recognition
DescriptionDuring infection the yeast Candida albicans undergoes a yeast-to-hyphal transition and secretes numerous proteins required for the invasion and damage of host tissues and the modulation of the host immune responses. Still little is known about the interplay of Candida albicans secreted proteins and the host adaptive immune system. For the characterization of the extracellular proteins of C. albicans and their ability to trigger a serological response in candidemia patients we applied 2D gel electrophoresis and LC-MS/MS-based approaches. These analyses identified the secretion of 101 different proteins from C. albicans yeast cells and 410 proteins from hyphal cells. Morphology-dependent changes of the secretome were mostly associated with proteins involved in cell wall organization, general stress response and the interplay with host immune cells. Our immunoproteomics workflow for the identification of secreted C. albicans protein antigens revealed a core set of 20 immunodominant specific anti- C. albicans protein antibodies. However, some secreted protein antigens showed cross-reactivity with sera from control groups without Candida infection. Glycoprotein staining of C. albicans hyphal secretome demonstrated that some secreted proteins are strongly glycosylated. Enzymatic deglycosylation of secreted proteins uncovered a significant contribution of glycan epitopes to the recognition of the secreted aspartyl protease Sap6 by IgG antibodies from patient sera.
HostingRepositoryPRIDE
AnnounceDate2022-03-03
AnnouncementXMLSubmission_2022-03-03_02:30:14.048.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD003201
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterThomas Krueger
SpeciesList scientific name: Candida albicans (Yeast); NCBI TaxID: 5476;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-11-16 23:25:08ID requested
12022-03-03 02:30:14announced
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biomedical
submitter keyword: Candida albicans, secretome, yeast-to-hypha transition, extracellular proteins, immunoproteomics, glycosylation
Contact List
Olaf Kniemeyer
contact affiliationLeibniz Institute for Natural Product Research and Infection Biology - Hans Knoell Institute Department of Molecular and Applied Microbiology Stress and Immunoproteomics Group Adolf-Reichwein-Str. 23, 07745, Jena, Germany
contact emailOlaf.Kniemeyer@leibniz-hki.de
lab head
Thomas Krueger
contact affiliationLeibniz Institute for Natural Product Research and Infection Biology - Hans Knoell Institute Jena
contact emailthomas.krueger@hki-jena.de
dataset submitter
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Dataset FTP location
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