PXD003201 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Immunoproteomic analysis of antibody responses to extracellular proteins of Candida albicans in serum of candidemia patients revealed glycosylation effects on protein antigen recognition |
Description | During infection the yeast Candida albicans undergoes a yeast-to-hyphal transition and secretes numerous proteins required for the invasion and damage of host tissues and the modulation of the host immune responses. Still little is known about the interplay of Candida albicans secreted proteins and the host adaptive immune system. For the characterization of the extracellular proteins of C. albicans and their ability to trigger a serological response in candidemia patients we applied 2D gel electrophoresis and LC-MS/MS-based approaches. These analyses identified the secretion of 101 different proteins from C. albicans yeast cells and 410 proteins from hyphal cells. Morphology-dependent changes of the secretome were mostly associated with proteins involved in cell wall organization, general stress response and the interplay with host immune cells. Our immunoproteomics workflow for the identification of secreted C. albicans protein antigens revealed a core set of 20 immunodominant specific anti- C. albicans protein antibodies. However, some secreted protein antigens showed cross-reactivity with sera from control groups without Candida infection. Glycoprotein staining of C. albicans hyphal secretome demonstrated that some secreted proteins are strongly glycosylated. Enzymatic deglycosylation of secreted proteins uncovered a significant contribution of glycan epitopes to the recognition of the secreted aspartyl protease Sap6 by IgG antibodies from patient sera. |
HostingRepository | PRIDE |
AnnounceDate | 2022-03-03 |
AnnouncementXML | Submission_2022-03-03_02:30:14.048.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD003201 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Thomas Krueger |
SpeciesList | scientific name: Candida albicans (Yeast); NCBI TaxID: 5476; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-11-16 23:25:08 | ID requested | |
⏵ 1 | 2022-03-03 02:30:14 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biomedical |
submitter keyword: Candida albicans, secretome, yeast-to-hypha transition, extracellular proteins, immunoproteomics, glycosylation |
Contact List
Olaf Kniemeyer |
contact affiliation | Leibniz Institute for Natural Product Research and Infection Biology - Hans Knoell Institute Department of Molecular and Applied Microbiology Stress and Immunoproteomics Group Adolf-Reichwein-Str. 23, 07745, Jena, Germany |
contact email | Olaf.Kniemeyer@leibniz-hki.de |
lab head | |
Thomas Krueger |
contact affiliation | Leibniz Institute for Natural Product Research and Infection Biology - Hans Knoell Institute Jena |
contact email | thomas.krueger@hki-jena.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003201
- Label: PRIDE project
- Name: Immunoproteomic analysis of antibody responses to extracellular proteins of Candida albicans in serum of candidemia patients revealed glycosylation effects on protein antigen recognition