PXD003189

PXD003189 is an original dataset announced via ProteomeXchange.

Title	Identification of FLS2 interacting proteins by co-immunoprecipitation and shot gun proteomics
Description	To identify immediate early signaling components in the FLS2 pathway we performed co-immunoprecipitation (co-IP) experiments with FLS2-GFP as bait in Arabidopsis. We immunoprecipitated FLS2-GFP with anti-GFP antibody coated beads and analyzed co-precipitated proteins by liquid chromatography tandem mass spectrometry (LC-MS/MS).
HostingRepository	PRIDE
AnnounceDate	2016-01-26
AnnouncementXML	Submission_2016-01-26_02:21:54.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003189
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jan Sklenar
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-11-16 01:40:24	ID requested
⏵ 1	2016-01-26 02:21:56	announced

Mithoe SC, Ludwig C, Pel MJ, Cucinotta M, Casartelli A, Mbengue M, Sklenar J, Derbyshire P, Robatzek S, Pieterse CM, Aebersold R, Menke FL, Attenuation of pattern recognition receptor signaling is mediated by a MAP kinase kinase kinase. EMBO Rep, 17(3):441-54(2016) [pubmed]

curator keyword: Biological

submitter keyword: Arabidopsis, immunity, pattern recognition receptor, FLS2, co-immunoprecipitation, protein-protein interaction, plasma membrane, signaling

Frank Menke
contact affiliation	Tha Sainsbury Laboratory
contact email	frank.menke@tsl.ac.uk
lab head
Jan Sklenar
contact affiliation	The Sainsbury Laboratory, Norwich, UK
contact email	jan.sklenar@tsl.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD003189

PRIDE project URI

• PRIDE
  1. PXD003189
     1. Label: PRIDE project
     2. Name: Identification of FLS2 interacting proteins by co-immunoprecipitation and shot gun proteomics