PXD003187

PXD003187 is an original dataset announced via ProteomeXchange.

Title	Human cTnT fragmentation in serum
Description	Cardiac troponin T (cTnT) is a highly cardiospecific protein commonly used in the diagnosis of acute myocardial infarction (AMI), but is subject to proteolytic degradation upon its release in the circulation. In this study, a targeted mass spectrometry assay was developed to detect peptides which are differentially present within the different degradation products. cTnT was spiked in human serum and incubated at 37 °C to induce proteolytic degradation. Isolation and fractionation of cTnT and its fragments from serum were performed using immunoprecipitation and SDS-PAGE. Bands migrating to 37 kDa (intact cTnT), 29 kDa (primary fragment), and 19, 18, and 16 kDa (secondary fragments) were excised, digested, and subsequently analysed using targeted selected ion monitoring on a UHPLC-coupled quadrupole-Orbitrap mass spectrometer. Sixteen precursor ions from a total of 11 peptides unique to cTnT were targeted. Precursor ions were detectable up until 1200 ng/L cTnT, which is a typical cTnT concentration after AMI. With tandem-MS and relative quantification, we proved the formation of cTnT fragments upon incubation in human serum and identified differentially present peptides in the fragment bands, indicative of N- and C-terminal proteolytic cleavage. These findings are of importance for the development of future cTnT assays, calibrators and quality control samples.
HostingRepository	PRIDE
AnnounceDate	2016-01-19
AnnouncementXML	Submission_2016-01-19_06:16:24.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sander Streng
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-11-16 01:24:29	ID requested
⏵ 1	2016-01-19 06:16:25	announced

Streng AS, de Boer D, Bouwman FG, Mariman EC, Scholten A, van Dieijen-Visser MP, Wodzig WK, Development of a targeted selected ion monitoring assay for the elucidation of protease induced structural changes in cardiac troponin T. J Proteomics, 136():123-32(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: cTnT, t-SIM

Will KWH Wodzig
contact affiliation	Central Diagnostic Laboratory Maastricht University Medical Centre Maastricht, the Netherlands
contact email	will.wodzig@mumc.nl
lab head
Sander Streng
contact affiliation	MUMC+
contact email	sander.streng@mumc.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003187
     1. Label: PRIDE project
     2. Name: Human cTnT fragmentation in serum