PXD003186

PXD003186 is an original dataset announced via ProteomeXchange.

Title	N-Myristoyltransferase inhibition in HeLa
Description	N-Myristoyltransferase (NMT) covalently attaches a C14-fatty acid to the N-terminal glycine of proteins and has been proposed as a therapeutic target in cancer. We used quantitative proteomics to map protein expression changes for more than 2700 proteins in response to treatment with an NMT inhibitor in HeLa cells, and observed down-regulation of proteins involved in cell cycle regulation, and up-regulation of many proteins involved in the endoplasmic reticulum stress response. This study defines the cellular response to NMT inhibition at the proteome level, and provides a knowledgebase for targeting specific cancers with NMT inhibitors, potentially in combination with other targeted agents.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:26:34.243.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emmanuelle Thinon
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-11-16 01:16:20	ID requested
1	2016-06-13 01:53:18	announced
⏵ 2	2024-10-22 04:26:35	announced	2024-10-22: Updated project metadata.

Thinon E, Morales-Sanfrutos J, Mann DJ, Tate EW, N-Myristoyltransferase Inhibition Induces ER-Stress, Cell Cycle Arrest, and Apoptosis in Cancer Cells. ACS Chem Biol, 11(8):2165-76(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: HeLa, N-myristoyltransferase inhibition, Q-exactive, spike-in SILAC

Ed Tate
contact affiliation	Department of Chemistry, Imperial College London, UK
contact email	e.tate@imperial.ac.uk
lab head
Emmanuelle Thinon
contact affiliation	The Francis Crick Institute
contact email	emmanuellethinon@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/06/PXD003186

PRIDE project URI

• PRIDE
  1. PXD003186
     1. Label: PRIDE project
     2. Name: N-Myristoyltransferase inhibition in HeLa