PXD003180

PXD003180 is an original dataset announced via ProteomeXchange.

Title	IP-MS of DCAF1/VprBP interacting proteins in mouse CD4 T cells
Description	DCAF1, also known as VprBP (HIV-1-viral-protein-r-binding-protein), is an evolutionary conserved substrate-binding subunit of CRL4 (Cul4a-Ddb1-Roc1) ubiquitin ligase complex. It is a cellular protein targeted by HIV-1 viral protein R (Vpr). DCAF1 modulates cellular response against HIV in macrophages, controls the survival and reprogramming of oocyte, and regulates G2/M transition. DCAF1 functions through diverse mechanisms including regulating protein poly-ubiquitination, mono-ubiquitination and phosphorylation. Nonetheless, whether and how DCAF1 controls the function of primary T cell, a major cell type infected by HIV, remains unknown. We found DCAF1 is required for cell size growth and cell cycle entry from quiescence. To investigate the underlying molecular mechanisms and identify the cellular factors that associate with DCAF1 in T cells, we analyzed DCAF1-interacting proteins in T cells using VprBP immunoprecipitation and mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2016-01-12
AnnouncementXML	Submission_2016-01-12_05:09:43.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yisong Wan
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-11-12 01:56:19	ID requested
⏵ 1	2016-01-12 05:09:44	announced

Guo Z, Kong Q, Liu C, Zhang S, Zou L, Yan F, Whitmire JK, Xiong Y, Chen X, Wan YY, DCAF1 controls T-cell function via p53-dependent and -independent mechanisms. Nat Commun, 7():10307(2016) [pubmed]

curator keyword: Biological

submitter keyword: DCAF1, VprBP, HIV-1, T cell

Yisong Y. Wan
contact affiliation	Department of Microbiology and Immunology, Lineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill
contact email	wany@email.unc.edu
lab head
Yisong Wan
contact affiliation	Lineberge Comprehensive Cancer Center Dept. of Microbiology and Immunology School of Medicine University of North Carolina at Chapel Hill
contact email	wany@email.unc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD003180

PRIDE project URI

• PRIDE
  1. PXD003180
     1. Label: PRIDE project
     2. Name: IP-MS of DCAF1/VprBP interacting proteins in mouse CD4 T cells