PXD003174

PXD003174 is an original dataset announced via ProteomeXchange.

Title	Tma108, a putative M1 aminopeptidase, defines specialized ribosomes in Saccharomyces cerevisiae
Description	Ribosome specialization is an emerging concept which challenges the common assumption that translation relies on a standardized molecular machinery. In this work, we demonstrate that Tma108, a yeast uncharacterized translation machinery-associated factor, defines a subpopulation of the cellular ribosomes specifically involved in the translation of less than 200 mRNAs encoding proteins with ATP or zinc binding domains. Ribonucleoparticle dissociation experiments support the fact that Tma108 directly interacts with the nascent protein chain. Comparative genomic analyses and molecular modeling point out Tma108 as an original M1 metallopeptidase with specific residues in the catalytic pocket which may explain its selectivity. The involvement of Tma108 in co-translational regulations is attested by the drastic perturbation of the subcellular localization of ATP2 mRNA, one of its targets, upon TMA108 inactivation. Tma108 is an unique example of a nascent chain-associated factor with high selectivity and illustrates the existence of specific translation-associated factors, besides RNA binding proteins.
HostingRepository	PRIDE
AnnounceDate	2016-09-27
AnnouncementXML	Submission_2016-09-26_21:46:06.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thibaut Léger
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; deamidated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-11-11 02:09:11	ID requested
⏵ 1	2016-09-26 21:46:08	announced

Delaveau T, Davoine D, Jolly A, Vallot A, Rouvi, è, re JO, Gerber A, Brochet S, Plessis M, Roquigny R, Merhej J, Leger T, Garcia C, Lelandais G, Laine E, Palancade B, Devaux F, Garcia M, Tma108, a putative M1 aminopeptidase, is a specific nascent chain-associated protein in Saccharomyces cerevisiae. Nucleic Acids Res, 44(18):8826-8841(2016) [pubmed]

curator keyword: Biological

submitter keyword: Affinity purification (AP-MS), yeast S. cerevisiae, ribosomes

Mathilde GARCIA
contact affiliation	Sorbonne Universités - UPMC Univ Paris 6 - UMR7238, Laboratoire de biologie computationnelle et quantitative, F-75006 PARIS, France
contact email	mathilde.garcia@upmc.fr
lab head
Thibaut Léger
contact affiliation	Institut Jacques Monod
contact email	leger@ijm.univ-paris-diderot.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003174
     1. Label: PRIDE project
     2. Name: Tma108, a putative M1 aminopeptidase, defines specialized ribosomes in Saccharomyces cerevisiae