PXD003163

PXD003163 is an original dataset announced via ProteomeXchange.

Title	Glycopeptide glycoforms analysis of mouse transferrin
Description	Collagen type II-induced arthritis (CIA) is a disease, which includes inflammatory and autoimmune reactions. Spleen protein extracts were subjected to 2D-DiGE and MS-MALDI-TOF/TOF analysis to identify proteins that were different in concentration ally expressed in CD38-KO and B6 WT mice with arthritis or with inflammation. Using multivariate analyses, in Col-II-immunized mice 20 differentially expressed spleen protein species were able to discriminate between WT and CD38-KO mice. Among them, multiple serotransferrin (Tf) species were identified. In contrast, in CFA/IFA-treated mice, the distinct protein profile, which discriminates between CD38-KO and WT mice, was unrelated with Tf. Unexpectedly, non-immunized CD38-KO mice showed a distinct proteome profile as compared with that in non-immunized WT mice, and again multiple protein species were identified as Tf. By using a LC-TOF-MS method to separate and detect Tf glycopeptide glycoforms in serum samples, differences in the Tf glycosylation pattern between CD38-KO and WT mice sera were found in both non-immunized and CIA+ mice. Data on Tf spots separated by 2-DE indicate differences in glycosylation related with NeuGc content. In summary, Tf changed significantly in its glycosylation pattern in mice with arthritis
HostingRepository	PRIDE
AnnounceDate	2015-12-10
AnnouncementXML	Submission_2015-12-10_04:01:47.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Albert Barroso
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	complex glycosylation
Instrument	6220 Time-of-Flight LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2015-11-09 02:46:47	ID requested
⏵ 1	2015-12-10 04:01:48	announced

Rosal-Vela A, Barroso A, Gim, é, nez E, Garc, í, a-Rodr, í, guez S, Longobardo V, Postigo J, Iglesias M, Lario A, Merino J, Merino R, Zubiaur M, Sanz-Nebot V, Sancho J, Identification of multiple transferrin species in the spleen and serum from mice with collagen-induced arthritis which may reflect changes in transferrin glycosylation associated with disease activity: The role of CD38. J Proteomics, 134():127-137(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: transferrin, mouse, spleen, serum, µLC-MS

Victoria Sanz Nebot
contact affiliation	Analytical Chemistry Department, University of Barcelona
contact email	vsanz@ub.edu
lab head
Albert Barroso
contact affiliation	University of Barcelona
contact email	albertbarroso@ub.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003163
     1. Label: PRIDE project
     2. Name: Glycopeptide glycoforms analysis of mouse transferrin