PXD003158

PXD003158 is an original dataset announced via ProteomeXchange.

Title	Effects of the antibacterial compund Allicin from garlic on Escherichia coli
Description	Allicin (diallyl thiosulfinate) from garlic is a highly potent natural antimicrobial substance. It inhibits growth of a variety of microorganisms, among them antibiotic resistant strains. Here, we show that upon exposure to allicin, growth of Escherichia coli is strongly inhibited. Growth inhibition coincides with a significant drop of total sulfhydryl concentrations and induction of the heat shock and oxidative stress response. In contrast to diamide, a potent inducer of disulfide stress, allicin does not induce cystine bond formation in proteins such as cytoplasmically expressed alkaline phosphatase PhoA. We identified and quantified the allicin-induced modification S-allylmercapto-cysteine for a set of cytoplasmic proteins by using a combination of label-free mass spectrometry and differential OxICAT labeling. Activity of isocitrate lyase AceA, an S-allylmercapto-modified candidate protein, is largely inhibited by allicin treatment in vivo. Our results indicate that the mode of action of allicin is a combination of disturbance of the redox balance and inactivation of crucial metabolic enzymes.
HostingRepository	PRIDE
AnnounceDate	2016-03-31
AnnouncementXML	Submission_2016-03-31_04:58:16.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lars Leichert
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2015-11-06 05:19:04	ID requested
⏵ 1	2016-03-31 04:58:17	announced

M, ü, ller A, Eller J, Albrecht F, Prochnow P, Kuhlmann K, Bandow JE, Slusarenko AJ, Leichert LI, Allicin Induces Thiol Stress in Bacteria through S-Allylmercapto Modification of Protein Cysteines. J Biol Chem, 291(22):11477-90(2016) [pubmed]

curator keyword: Biological

submitter keyword: Allicin, S-allylmercapto modification, cysteine, Escherichia coli

Lars I Leichert
contact affiliation	Ruhr-Universität Bochum Institute for Biochemistry and Pathobiochemistry Microbial Biochemistry Universitätsstrasse 150 44780 Bochum, Germany
contact email	lars.leichert@ruhr-uni-bochum.de
lab head
Lars Leichert
contact affiliation	Ruhr-Universität Bochum
contact email	lars.leichert@ruhr-uni-bochum.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003158
     1. Label: PRIDE project
     2. Name: Effects of the antibacterial compund Allicin from garlic on Escherichia coli