PXD003156

PXD003156 is an original dataset announced via ProteomeXchange.

Title	Gel-free/label-free proteomic analysis of developing rice grains under heat stress
Description	High temperature markedly reduces the yields and quality of rice grains. To identify the mechanisms underlying heat stress-induced responses in rice grains, proteomic technique was used. Khao Dawk Mali 105 rice grains at the milky, doughy, and mature stages of development after flowering were treated at 40 °C for 3 days. Aromatic compounds were decreased in rice grains under heat stress. The protein abundance involved in glycolysis and tricarboxylic acid cycle, including glyceraldehyde 3-phosphate dehydrogenase and citrate synthase, was changed in milky and doughy grains after heat treatment; however, no changes in mature grains. The abundance involved in amino acid metabolism was increased in doughy grains, but decreased in milky grains. In addition, the abundance involved in starch and sucrose metabolism, such as starch synthase, ADP-glucose pyrophosphorylase, granule-bound starch synthase, and alpha amylase, was decreased in milky grains, but increased in doughy grains. A number of redox homeostasis-related proteins, such as ascorbate peroxidase and peroxiredoxin, were increased in developing rice grains treated with heat stress. These results suggest that in response to heat stress, the abundance of numerous proteins involved in redox homeostasis and carbohydrate biosynthetic pathways may play a major role in the development of KDML105 rice grains.
HostingRepository	PRIDE
AnnounceDate	2015-12-17
AnnouncementXML	Submission_2015-12-17_05:36:30.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	xiaojian Yin
SpeciesList	scientific name: Rice(O. sativa); NCBI TaxID: 4530;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-11-06 02:20:08	ID requested
⏵ 1	2015-12-17 05:36:31	announced

Timabud T, Yin X, Pongdontri P, Komatsu S, Gel-free/label-free proteomic analysis of developing rice grains under heat stress. J Proteomics, 133():1-19(2016) [pubmed]

curator keyword: Biological

submitter keyword: Rice, Heat stress, Proteomics, Developing rice grain

Setsuko Komatsu
contact affiliation	NARO Institute of Crop Science
contact email	skomatsu@affrc.go.jp
lab head
xiaojian Yin
contact affiliation	University of Tsukuba
contact email	xiaojian@affrc.go.jp
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003156
     1. Label: PRIDE project
     2. Name: Gel-free/label-free proteomic analysis of developing rice grains under heat stress