PXD003154

PXD003154 is an original dataset announced via ProteomeXchange.

Title	Proteomics of Tomato Trichome Metabolism
Description	Glandular trichomes (GT) are specialized cell factories that have the capacity to produce large amounts of metabolites which can amount to over 10% of the leaf dry weight. The specific expression of secondary metabolite pathways in glandular trichomes has facilitated their elucidation. However, little is known about the connection between central carbon and specialized metabolism in these cells. To address this question, we used the type VI glandular trichomes from a cultivated (Solanum lycopersicum LA4024) and a wild tomato accession (Solanum habrochaites LA1777) as a model. Our study is based on metabolomics, transcriptomics, proteomics and 13C-labeling datasets of trichome and leaves samples. This comparative analysis allowed us to identify specific features of trichomes in comparison to leaves. Tomato type VI trichomes are photosynthetic but acquire their carbon essentially from the leaves in the form of sucrose. Relatively high expression levels of photosystem genes and comparatively low expression of Calvin-cycle and photorespiration genes indicate that photosynthesis is used primarily to produce energy and reducing power to drive the high metabolic activity. High levels of polyunsaturated fatty acids, oxylipins, and of oxidized glutathione and high transcript and protein levels of associated enzymes further indicate a distinct capacity of GT to cope with oxidative stress. Enzymes which are involved in the supply of key precursors for the production of terpenoids both from the cytosol and in the chloroplasts are markedly increased. Finally, some enzymes that are typically associated with C4-photosynthesis are highly expressed in trichomes, suggesting that CO2 recycling processes may increase the efficiency of C usage in these cells. Based on this information we propose a model on how the glandular trichomes achieve high metabolic efficiency.
HostingRepository	PRIDE
AnnounceDate	2017-07-04
AnnouncementXML	Submission_2017-07-04_05:46:26.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003154
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Wolfgang Hoehenwarter
SpeciesList	scientific name: Solanum lycopersicum; NCBI TaxID: 4081;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-11-05 05:48:22	ID requested
⏵ 1	2017-07-04 05:46:27	announced

Balcke GU, Bennewitz S, Bergau N, Athmer B, Henning A, Majovsky P, Jim, é, nez-G, ó, mez JM, Hoehenwarter W, Tissier A, Multi-Omics of Tomato Glandular Trichomes Reveals Distinct Features of Central Carbon Metabolism Supporting High Productivity of Specialized Metabolites. Plant Cell, 29(5):960-983(2017) [pubmed]

curator keyword: Biological

submitter keyword: Tomato, Trichomes

Wolfgang Hoehenwarter
contact affiliation	Leibniz Institute of Plant Biochemistry
contact email	wolfgang.hoehenwarter@ipb-halle.de
lab head
Wolfgang Hoehenwarter
contact affiliation	Leibniz Institute of Plant Biochemistry
contact email	wolfgang.hoehenwarter@ipb-halle.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003154
     1. Label: PRIDE project
     2. Name: Proteomics of Tomato Trichome Metabolism