PXD003149

PXD003149 is an original dataset announced via ProteomeXchange.

Title	Synthesis of chlorophyll-binding proteins in a fully-segregated ∆ycf54 strain of the cyanobacterium Synechocystis PCC 6803
Description	The MS analysis is part of a wider study into chlorophyll biosynthesis. There is previous evidence, from both pulldown and CN-PAGE/immunoblot analyses that the enzyme Mg-protoporphyrin IX methylester cyclase (Cyc1), which catalyses the formation of the the fifth (E) ring in chlorophyll, associates with a protein of unknown function called Ycf54. There is also evidence for the association of Cyc1 with other enzymes in the chlorophyll biosynthetic pathway, eg. POR, ChlP and DVR. In the MS part of this study, FLAG-tagged Cyc1 was used to capture proteins from a Synechocystis cell lysate, with a comparison of FLAG-Cyc1 strains against wild-type and Ycf54 deletion backgrounds. MS-based quantification of the amounts of POR, ChlP and DVR relative to the Cyc1 bait supported evidence by immunoblotting of a significant reduction in these co-isolated proteins in the Ycf54 deletion strain.
HostingRepository	PRIDE
AnnounceDate	2016-04-01
AnnouncementXML	Submission_2016-03-31_16:23:36.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003149
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Philip Jackson
SpeciesList	scientific name: Synechocystis sp. (strain PCC 6803 / Kazusa); NCBI TaxID: 1111708;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive; maXis

Revision	Datetime	Status	ChangeLog Entry
0	2015-11-05 02:28:32	ID requested
⏵ 1	2016-03-31 16:23:37	announced

Hollingshead S, Kope, č, n, á J, Armstrong DR, Bu, č, insk, á L, Jackson PJ, Chen GE, Dickman MJ, Williamson MP, Sobotka R, Hunter CN, ycf54 Strain of the Cyanobacterium Synechocystis PCC 6803. Front Plant Sci, 7():292(2016) [pubmed]

curator keyword: Biological

submitter keyword: Ycf54, Synechocystis 6803, chlorophyll, photosystem II, protochlorophyllide, Mg-protoporphyrin IX methylester cyclase

Christopher Neil Hunter
contact affiliation	Department of Molecular Biology and Biotechnology
contact email	c.n.hunter@sheffield.ac.uk
lab head
Philip Jackson
contact affiliation	Department of Chemical and Biological Engineering
contact email	p.j.jackson@sheffield.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003149
     1. Label: PRIDE project
     2. Name: Synthesis of chlorophyll-binding proteins in a fully-segregated ∆ycf54 strain of the cyanobacterium Synechocystis PCC 6803