PXD003148

PXD003148 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	C1QBP mitochondrial interactome
Description	The study of protein-protein interactions is an essential process to understand the biological functions of proteins and the underlying mechanisms. Co-immunoprecipitation coupled with mass spectrometry (CoIP-MS) is one of the most extensively used high-throughput techniques to discover novel protein-protein interactions. However, the traditional CoIP process uses whole cell lysate, disrupts cellular organization, and leads to potential false positives by inducing artificial protein-protein interactions. Here we have developed a strategy by combining subcellular fractionation (SCF) with CoIP -MS to study the interacting proteins of the complement component 1, q subcomponent binding protein (C1QBP) in the mitochondria. Using this method, a novel C1QBP interacting protein, dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial (DLAT) was identified and validated.
HostingRepository	MassIVE
AnnounceDate	2021-02-01
AnnouncementXML	Submission_2021-02-01_13:37:11.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ruibing Chen
SpeciesList	scientific name: homo sapiens;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Discovery

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2015-11-04 21:15:08	ID requested
⏵ 1	2021-02-01 13:37:12	announced

Publication List

Chen R, Xiao M, Gao H, Chen Y, Li Y, Liu Y, Zhang N, Identification of a novel mitochondrial interacting protein of C1QBP using subcellular fractionation coupled with CoIP-MS. Anal Bioanal Chem, 408(6):1557-64(2016) [pubmed]

Chen R, Xiao M, Gao H, Chen Y, Li Y, Liu Y, Zhang N, Identification of a novel mitochondrial interacting protein of C1QBP using subcellular fractionation coupled with CoIP-MS. Anal Bioanal Chem, 408(6):1557-64(2016) [pubmed]

Keyword List

submitter keyword: C1QBP, AP-MS, protein-protein interaction, mitochondria

submitter keyword: C1QBP, AP-MS, protein-protein interaction, mitochondria

Contact List

Ruibing Chen
lab head
Ruibing Chen
contact affiliation	Tianjin Medical University
contact email	chenruibing@tijmu.edu.cn
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000079369/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000079369/