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PXD003144

PXD003144 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMeasuring C2C12 Myotube Protein Expression Changes in Inflammation using Punch-P
DescriptionSelective identification of newly synthesized proteins provides insight on biological processes that depend on the continued synthesis of specific proteins to maintain homeostasis as well as those that are up- or down-regulated in response to changing needs. We used puromycin-associated nascent chain proteomics (PUNCH-P) to characterize new protein synthesis in C2C12 myotubes and changes resulting from exposure to a combination of lipopolysaccharide and interferon-(LPS/IFN) for either a short (4h) or prolonged (16h) time period. We identified sequences of nascent polypeptide chains belonging to a total of 1,523 proteins and report their detection within three independent samples for each growth condition. The identified proteins correspond to approximately 15% of presently known proteins in C2C12 myotubes and are enriched in specific cellular components and pathways. A subset of these identified proteins have functional characteristics and were identified only in treated samples, consistent with new protein synthesis in response to LPS/IFN. Thus, the identification of proteins from their nascent polypeptide chains provides a resource to analyze the role of newly synthesizing proteins in protein homeostasis and proteome responses in C2C12 myotubes.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:34:05.919.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBruce Stanley
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListS-carboxamidomethyl-L-cysteine
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-11-04 01:37:34ID requested
12022-02-28 02:43:49announced
22024-10-22 05:34:06announced2024-10-22: Updated project metadata.
Publication List
Coleman CS, Stanley BA, Lang CH, . Inflammation, 45(3):1313-1331(2022) [pubmed]
10.1007/s10753-022-01622-3;
Keyword List
curator keyword: Biomedical
submitter keyword: LPS,mouse, inflammatio, interferon gamma, 5600 TripleTOF, muscle, myotubes
Contact List
Bruce A. Stanley
contact affiliationSection of Research Resources H093 The Pennsylvania State University College of Medicine 500 University Drive Hershey, PA 17033-0850 USA
contact emailbstanley@psu.edu
lab head
Bruce Stanley
contact affiliationPenn State College of Medicine
contact emailbstanley@psu.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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