PXD003130

PXD003130 is an original dataset announced via ProteomeXchange.

Title	Sexual crossing of thermophilic fungus Myceliophthora heterothallica improved enzymatic degradation of sugar beet pulp
Description	Enzymatic degradation of plant biomass requires a complex mixture of many different enzymes. Like most fungi, thermophilic Myceliophthora species therefore have a large set of enzymes targeting different linkages in plant polysaccharides. The majority of these enzymes have not been functionally characterized and their role in plant biomass degradation is unknown. This study describes a strategy using sexual crossing and screening with the thermophilic fungus Myceliophthora heterothallica to identify specific enzymes associated with improved sugar beet pulp saccharification.Two genetically diverse M. heterothallica strains CBS 203.75 and CBS 663.74 were used to generate progenies with improved growth on sugar beet pulp. One progeny, named SBP.F1.2.11, had a different genetic pattern from the parental strains, and had improved saccharification activity after growth on 3% sugar beet pulp. Exo-proteome analysis of progeny and parental strains after 7 days growth on sugar beet pulp showed that only 17 of the 133 secreted CAZy enzymes were more abundant in progeny SBP.F1.2.11. Particularly one enzyme belonging to the carbohydrate esterase family 5 (CE5) was more present in SBP.F1.2.11. This CE5-CBM1 enzyme, named as Axe1, was phylogenetically related to acetyl xylan esterases.
HostingRepository	PRIDE
AnnounceDate	2016-02-25
AnnouncementXML	Submission_2016-02-25_05:08:24.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maria Victoria Aguilar Pontes
SpeciesList	scientific name: Myceliophthora heterothallica; NCBI TaxID: 1149786;
ModificationList	monohydroxylated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-11-02 02:33:00	ID requested
⏵ 1	2016-02-25 05:08:26	announced

Aguilar-Pontes MV, Zhou M, van der Horst S, Theelen B, de Vries RP, van den Brink J, Sexual crossing of thermophilic fungus Myceliophthora heterothallica improved enzymatic degradation of sugar beet pulp. Biotechnol Biofuels, 9():41(2016) [pubmed]

curator keyword: Biological

submitter keyword: Myceliophthora heterothallica, progeny, plant biomass, sugar beep pulp, saccharification, CAZy enzymes

Ronald P. de Vries
contact affiliation	Fungal Physiology, CBS-KNAW Fungal Biodiversity Centre Institute of the Royal Netherlands Academy of Arts and Sciences(KNAW) Fungal Molecular Physiology, Utrecht University Utrecht, The Netherlands
contact email	r.devries@cbs.knaw.nl
lab head
Maria Victoria Aguilar Pontes
contact affiliation	CBS-KNAW-Utrecht University
contact email	v.aguilar@cbs.knaw.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/02/PXD003130

PRIDE project URI

• PRIDE
  1. PXD003130
     1. Label: PRIDE project
     2. Name: Sexual crossing of thermophilic fungus Myceliophthora heterothallica improved enzymatic degradation of sugar beet pulp