PXD003126

PXD003126 is an original dataset announced via ProteomeXchange.

Title	Proteomic investigation of a mixed microbial culture synthesizing polyhydroxyalkanoates from fermented dairy manure under aerobic feast-famine conditions
Description	Polyhydroxyalkanoates (PHAs) are bio-based, biodegradable polyesters that can be produced from organic-rich waste streams using mixed microbial cultures. To maximize PHA production, mixed microbial cultures may be enriched for PHA-producing bacteria with a high storage capacity through the imposition of cyclic, aerobic feast-famine conditions in a sequencing batch reactor (SBR). Though enrichment SBRs have been extensively investigated a bulk solutions-level, little evidence at the proteome level is available to describe the observed SBR behavior to guide future SBR optimization strategies. As such, the purpose of this investigation was to characterize proteome dynamics of a mixed microbial culture in an SBR operated under aerobic feast-famine conditions using fermented dairy manure as the feedstock for PHA production. At the beginning of the SBR cycle, excess PHA precursors were provided to the mixed microbial culture (i.e., feast), after which followed a long duration devoid of exogenous substrate (i.e., famine). Two-dimensional electrophoresis was used to separate protein mixtures during a complete SBR cycle, and proteins of interest were identified.
HostingRepository	PRIDE
AnnounceDate	2017-01-31
AnnouncementXML	Submission_2017-01-31_04:56:49.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003126
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andrea Hanson
SpeciesList	scientific name: environmental samples; NCBI TaxID: 135062;
ModificationList	Deamidated; Oxidation; Carbamidomethyl
Instrument	Q-Tof Premier

Revision	Datetime	Status	ChangeLog Entry
0	2015-11-02 01:06:56	ID requested
⏵ 1	2017-01-31 04:56:50	announced

Hanson AJ, Guho NM, Paszczynski AJ, Coats ER, Community proteomics provides functional insight into polyhydroxyalkanoate production by a mixed microbial culture cultivated on fermented dairy manure. Appl Microbiol Biotechnol, 100(18):7957-76(2016) [pubmed]

curator keyword: Metaproteomics, Biological

submitter keyword: mixed microbial culture, polyhydroxyalkanoates, two-dimensional electrophoresis

Erik Coats
contact affiliation	University of Idaho, Department of Civil Engineering
contact email	ecoats@uidaho.edu
lab head
Andrea Hanson
contact affiliation	University of Idaho
contact email	hans2973@vandals.uidaho.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003126
     1. Label: PRIDE project
     2. Name: Proteomic investigation of a mixed microbial culture synthesizing polyhydroxyalkanoates from fermented dairy manure under aerobic feast-famine conditions