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PXD003124

PXD003124 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDog tear film proteome in-depth analysis
DescriptionIn this study, mass spectrometry was used to explore the canine tear proteome. Tear samples were obtained from six healthy dogs, and one-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (1D SDS-PAGE) was used as a first step to separate intact proteins into 17 bands. Each fraction was then trypsin digested and analysed by matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF-MS/MS) to characterize the protein components in each fraction. In total, 125 tear proteins were identified, with MCA (Major Canine Allergen), Serum albumin, UPF0557 protein C10orf119 homolog, Collagen alpha-2(I) chain, Tyrosine -protein kinase Fer, Keratine type II cytoskeletal, Beta-crystallin B2, Interleukin-6 and Desmin occuring as the most confident ones with the highest scores. The results showed that the proteomic strategy used in this study was successful in the analysis of the dog tear proteome. To the best of our knowledge, this study is the first to report the comprehensive proteome profile of tears from healthy dogs by 1D SDS PAGE and MALDI-TOF.
HostingRepositoryPRIDE
AnnounceDate2015-11-23
AnnouncementXMLSubmission_2016-01-05_14:45:36.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTomasz Paweł Banach
SpeciesList scientific name: Canis familiaris (Dog) (Canis lupus familiaris); NCBI TaxID: 9615;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
Instrumentultraflex
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-10-30 03:50:13ID requested
12015-11-23 08:41:02announced
22016-01-05 14:45:37announcedUpdated publication reference for PubMed record(s): 26701646.
Publication List
Winiarczyk M, Winiarczyk D, Banach T, Adaszek L, Madany J, Mackiewicz J, Pietras-Ozga D, Winiarczyk S, Dog Tear Film Proteome In-Depth Analysis. PLoS One, 10(12):e0144242(2015) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: dog, tear fluid, proteome, SDS-Page, MALDI
Contact List
prof. Stanisław Winiarczyk
contact affiliationDepartment of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine
contact emailgenp53@interia.pl
lab head
Tomasz Paweł Banach
contact affiliationUniversity of Life Sciences in Lublin
contact emailtomasz.banach@up.lublin.pl
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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