PXD003124 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Dog tear film proteome in-depth analysis |
Description | In this study, mass spectrometry was used to explore the canine tear proteome. Tear samples were obtained from six healthy dogs, and one-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (1D SDS-PAGE) was used as a first step to separate intact proteins into 17 bands. Each fraction was then trypsin digested and analysed by matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF-MS/MS) to characterize the protein components in each fraction. In total, 125 tear proteins were identified, with MCA (Major Canine Allergen), Serum albumin, UPF0557 protein C10orf119 homolog, Collagen alpha-2(I) chain, Tyrosine -protein kinase Fer, Keratine type II cytoskeletal, Beta-crystallin B2, Interleukin-6 and Desmin occuring as the most confident ones with the highest scores. The results showed that the proteomic strategy used in this study was successful in the analysis of the dog tear proteome. To the best of our knowledge, this study is the first to report the comprehensive proteome profile of tears from healthy dogs by 1D SDS PAGE and MALDI-TOF. |
HostingRepository | PRIDE |
AnnounceDate | 2015-11-23 |
AnnouncementXML | Submission_2016-01-05_14:45:36.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Tomasz Paweł Banach |
SpeciesList | scientific name: Canis familiaris (Dog) (Canis lupus familiaris); NCBI TaxID: 9615; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | ultraflex |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-10-30 03:50:13 | ID requested | |
1 | 2015-11-23 08:41:02 | announced | |
⏵ 2 | 2016-01-05 14:45:37 | announced | Updated publication reference for PubMed record(s): 26701646. |
Publication List
Winiarczyk M, Winiarczyk D, Banach T, Adaszek L, Madany J, Mackiewicz J, Pietras-Ozga D, Winiarczyk S, Dog Tear Film Proteome In-Depth Analysis. PLoS One, 10(12):e0144242(2015) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: dog, tear fluid, proteome, SDS-Page, MALDI |
Contact List
prof. Stanisław Winiarczyk |
contact affiliation | Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine |
contact email | genp53@interia.pl |
lab head | |
Tomasz Paweł Banach |
contact affiliation | University of Life Sciences in Lublin |
contact email | tomasz.banach@up.lublin.pl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003124
- Label: PRIDE project
- Name: Dog tear film proteome in-depth analysis