PXD003107

PXD003107 is an original dataset announced via ProteomeXchange.

Title	Combining Untargeted and Targeted Proteomic Strategies for Discrimination and Quantification of Cashmere Fibers
Description	Cashmere is regarded as a specialty and luxury fiber due to its scarcity and high economic value. For fiber quality assessment, it is technically very challenging to distinguish and quantify the cashmere fiber from yak or wool fibers because of their highly similar physical appearance and substantial protein sequence homology. To address this issue, we propose a workflow combining untargeted and targeted proteomics strategies for selecting, verifying and quantifying biomarkers for cashmere textile authentication. Untargeted proteomic surveys were first applied to identify 174, 157, and 156 proteins from cashmere, wool and yak fibers respectively. After marker selection at different levels, peptides turned out to afford much higher selectivity than proteins for fiber species discrimination. Collective use of these peptide makers allowed us to discriminate and quantify cashmere fibers in commercial finished fabrics that have undergone heavy chemical treatments. Cashmere proportion measurement in fabric samples using our proteomic approach was in good agreement with results from traditional light microscopy, yet our method can be more readily standardized to become an objective and robust assay for assessing authenticity of fibers and textiles.
HostingRepository	PRIDE
AnnounceDate	2018-10-25
AnnouncementXML	Submission_2018-10-25_01:15:13.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guo Yufeng
SpeciesList	scientific name: Capra hircus (Goat); NCBI TaxID: 9925; scientific name: Bos mutus grunniens (Wild yak) (Bos grunniens); NCBI TaxID: 30521; scientific name: Ovis aries (Sheep); NCBI TaxID: 9940;
ModificationList	monohydroxylated residue; alpha-amino dimethylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2015-10-26 03:53:31	ID requested
⏵ 1	2018-10-25 01:15:14	announced

Li S, Zhang Y, Wang J, Yang Y, Miao C, Guo Y, Zhang Z, Cao Q, Shui W, Combining Untargeted and Targeted Proteomic Strategies for Discrimination and Quantification of Cashmere Fibers. PLoS One, 11(1):e0147044(2016) [pubmed]

curator keyword: Biological

submitter keyword: Fiber, keratin, keratin-associated protein, biomarker, proteomics, LC-MS

Shui Wenqing
contact affiliation	Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
contact email	shui_wq@tib.cas.cn
lab head
Guo Yufeng
contact affiliation	Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
contact email	guo_yf@tib.cas.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD003107

PRIDE project URI

• PRIDE
  1. PXD003107
     1. Label: PRIDE project
     2. Name: Combining Untargeted and Targeted Proteomic Strategies for Discrimination and Quantification of Cashmere Fibers