PXD003105

PXD003105 is an original dataset announced via ProteomeXchange.

Title	Identification of Glycosylphosphatidylinositol Anchored Proteins and ω-sites Using TiO2-Based Affinity Purification
Description	Glycosylphosphatidylinositol anchored proteins (GPI-APs) in the outer leaflet of the membrane microdomains, commonly referred to as lipid rafts, play important roles in many biological processes. To enable large-scale site-specific analysis of GPI anchoring, we developed a method for identification of ω-sites by mass spectrometry by combining titanium dioxide-based affinity purification and hydrogen fluoride treatment. This method was able to identify ~3-fold more GPI-APs than our previous method: the new technique identified a total of 73 ω-sites derived from 49 GPI-APs. In 13 of the 49 GPI-APs identified, the GPI-anchor attached to multiple amino acids in the C-terminal site, yielding a variety of different protein species. This method allows us to simultaneously identify many GPI-AP protein species with different ω-sites.
HostingRepository	PRIDE
AnnounceDate	2017-08-02
AnnouncementXML	Submission_2017-08-02_06:49:33.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003105
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yusuke Masuishi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-10-26 01:59:56	ID requested
1	2016-05-27 05:35:22	announced
⏵ 2	2017-08-02 06:49:34	announced	Updated project metadata.

Masuishi Y, Kimura Y, Arakawa N, Hirano H, -sites in cancer cell lines. Data Brief, 7():1302-5(2016) [pubmed]

curator keyword: Technical

submitter keyword: GPI anchor

Yusuke Masuishi
contact affiliation	Yusuke Masuishi, Yokohama City University Hisashi Hirano, Graduate School of Medical Life Science and Advanced Medical Research Center Yokohama City University, Yokohama Kanagawa, Japan ( lab head )
contact email	ym5822@tsurumi.yokohama-cu.ac.jp
lab head
Yusuke Masuishi
contact affiliation	Yokohama City University
contact email	ym5822@tsurumi.yokohama-cu.ac.jp
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD003105

PRIDE project URI

• PRIDE
  1. PXD003105
     1. Label: PRIDE project
     2. Name: Identification of Glycosylphosphatidylinositol Anchored Proteins and ω-sites Using TiO2-Based Affinity Purification