PXD003102 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Sulfolobus islandicus HCD+ETD, including LFQ |
Description | Protein posttranslational methylation and acetylations have been reported to occur in archaea, including members of the genus Sulfolobus, but have not been characterized on a proteome-wide scale. Sulfolobus chromatin proteins are known to be methylated and acetylated on lysine side chains, resembling eukaryotic histones in this respect. We utilized bottom-up and top-down proteomic approaches to perform a global and deep methylation study in the hyperthermoacidophylic archaeon S. islandicus LAL 14/1 with a particular focus on chromatin proteins. Without specific enrichment, 1931 methylation sites on 731 protein were found by bottom-up proteomic analysis. The dynamics of protein methylation has been investigated on 424-526 proteins throughout 3 cell culture growth stages. We also confirm the relaxed specificity and steady abundance of the previously described methyltransferase aKMT4 which is implicated in the massive proteome methylation. We also detected an abundant N-terminal acetylation of S. islandicus proteins, with more than one third of the detected N-terminal peptides being acetylated |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:48:50.701.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Egor Vorontsov |
SpeciesList | scientific name: Sulfolobus islandicus LAL14/1; NCBI TaxID: 1241935; |
ModificationList | monomethylated residue; acetylated residue; monohydroxylated residue |
Instrument | Orbitrap Fusion ETD |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-10-23 01:28:05 | ID requested | |
1 | 2022-03-01 10:05:52 | announced | |
⏵ 2 | 2023-11-14 08:48:51 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: strong cation exchange,Archaea, HCD, Label-free quantitation, ETD, Sulfolobus islandicus |
Contact List
Julia Chamot-Rooke |
contact affiliation | Structural Mass Spectrometry and Proteomics Unit, Structural Biology and Chemistry Department, Institut Pasteur, 75015 Paris, France |
contact email | julia.chamot-rooke@pasteur.fr |
lab head | |
Egor Vorontsov |
contact affiliation | Proteomics Core Facility, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden |
contact email | yegor.msu@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD003102 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003102
- Label: PRIDE project
- Name: Sulfolobus islandicus HCD+ETD, including LFQ