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PXD003102

PXD003102 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSulfolobus islandicus HCD+ETD, including LFQ
DescriptionProtein posttranslational methylation and acetylations have been reported to occur in archaea, including members of the genus Sulfolobus, but have not been characterized on a proteome-wide scale. Sulfolobus chromatin proteins are known to be methylated and acetylated on lysine side chains, resembling eukaryotic histones in this respect. We utilized bottom-up and top-down proteomic approaches to perform a global and deep methylation study in the hyperthermoacidophylic archaeon S. islandicus LAL 14/1 with a particular focus on chromatin proteins. Without specific enrichment, 1931 methylation sites on 731 protein were found by bottom-up proteomic analysis. The dynamics of protein methylation has been investigated on 424-526 proteins throughout 3 cell culture growth stages. We also confirm the relaxed specificity and steady abundance of the previously described methyltransferase aKMT4 which is implicated in the massive proteome methylation. We also detected an abundant N-terminal acetylation of S. islandicus proteins, with more than one third of the detected N-terminal peptides being acetylated
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:48:50.701.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterEgor Vorontsov
SpeciesList scientific name: Sulfolobus islandicus LAL14/1; NCBI TaxID: 1241935;
ModificationListmonomethylated residue; acetylated residue; monohydroxylated residue
InstrumentOrbitrap Fusion ETD
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-10-23 01:28:05ID requested
12022-03-01 10:05:52announced
22023-11-14 08:48:51announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biological
submitter keyword: strong cation exchange,Archaea, HCD, Label-free quantitation, ETD, Sulfolobus islandicus
Contact List
Julia Chamot-Rooke
contact affiliationStructural Mass Spectrometry and Proteomics Unit, Structural Biology and Chemistry Department, Institut Pasteur, 75015 Paris, France
contact emailjulia.chamot-rooke@pasteur.fr
lab head
Egor Vorontsov
contact affiliationProteomics Core Facility, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
contact emailyegor.msu@gmail.com
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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