PXD003099

PXD003099 is an original dataset announced via ProteomeXchange.

Title	Modifications of fungal membrane proteins profile under pathogenicity induction: a proteomic analysis of Botrytis cinerea membranome.
Description	Botrytis cinerea is a model phytopathogenic fungus that infects more than 200 different relevant crops, thus being classified among the top ten fungal plant diseases. B. cinerea exhibits a wide arsenal of tools to infect plant tissues. Most of these factors are related to signal transduction cascades, in which membrane proteins play a key role as a bridge between environmental conditions and intracellular molecular processes. This work describes the first proteomic approach to study the membranome of B. cinerea under different pathogenicity condition induced by using different plant-based elicitors: Glucose and Tomato Cell Wall (TCW). A discovery proteomics analysis of membrane protein extracts was carried out by mass spectrometry. A total of 2,794 proteins were successfully identified, and approximately 46% of them could be classified as membrane proteins based on the presence of transmembrane regions and covalent post-translational modifications. Further analyses showed significant differences in the fungal membranome composition depending on the available carbon source: 804 proteins were exclusively identified when the fungus was cultured in the presence of glucose as sole carbon source, and 251 proteins were exclusively identified in the presence of TCW. Besides, among the 1737 common proteins, a subset of 898 proteins presented clear differences in their abundance and in order to clarify the specific changes produced in the membranome, proteins were categorized according to their gene ontology annotation shown different profiles. As a result of this work, we present here the first description of the membranome of B. cinerea, which will certainly provide a better understanding of this important subproteome in B. cinerea.
HostingRepository	PRIDE
AnnounceDate	2016-07-11
AnnouncementXML	Submission_2016-07-11_06:31:58.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cristina Chiva
SpeciesList	scientific name: Botryotinia fuckeliana (Noble rot fungus) (Botrytis cinerea); NCBI TaxID: 40559; scientific name: Capsaspora owczarzaki; NCBI TaxID: 192875;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-10-22 06:51:52	ID requested
⏵ 1	2016-07-11 06:31:59	announced

Li, ñ, eiro E, Chiva C, Cantoral JM, Sabid, ó E, Fern, á, ndez-Acero FJ, Modifications of fungal membrane proteins profile under pathogenicity induction: A proteomic analysis of Botrytis cinerea membranome. Proteomics, 16(17):2363-76(2016) [pubmed]

curator keyword: Biological

submitter keyword: Botritys cinerea, membranome, proteomics, phytopathogenicity

Francisco Javier Fernández-Acero
contact affiliation	Andalusian Center for Grape and Grapevine Research. CeiA3. Marine and Environmental Sciences Faculty. University of Cadiz. Puerto Real, 11510, Cádiz, Spain.
contact email	franciscojavier.fernandez@uca.es
lab head
Cristina Chiva
contact affiliation	Center of genomic regulation
contact email	chiva.cristina@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003099
     1. Label: PRIDE project
     2. Name: Modifications of fungal membrane proteins profile under pathogenicity induction: a proteomic analysis of Botrytis cinerea membranome.